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VINCENT J. PILEGGI, NORMAN D. LEE, ORVILLE J. GOLUB, RICHARD J. HENRY, DETERMINATION OF IODINE COMPOUNDS IN SERUM. I. SERUM HYROXINE IN THE PRESENCE OF SOME IODINE CONTAMINANTS, The Journal of Clinical Endocrinology & Metabolism, Volume 21, Issue 10, 1 October 1961, Pages 1272–1279, https://doi.org/10.1210/jcem-21-10-1272
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A column method utilizing the anion-exchange rosin Dowex-1 is described for the determination of thyroxine in serum. Values comparable to those for butanol-extractable iodine were obtained, and additions of inorganic iodine or iodotyrosines did not affect the reliability. Analyses of sera containing in vitro additions of organic iodine compounds which interfere with the PBI and in most cases the BE1, gave results which suggested the possible usefulness of the column method. Furthermore, the pattern of thyroxine elution with the column method, as determined by the iodine content of 3 successive 1.0-ml. fractions, was helpful in predicting the validity or nonvalidity of the result. When the BEI and columnte chniques were applied to cases in which elevated serum PBI values did not correlate with the clinical findings, there was some evidence for better specificity of the column method. Neither of the methods was helpful in most cases involving both inorganic iodine and PBI values in excess of 25 μg. per 100 ml.
