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CARL A. GEMZELL, DEMONSTRATION OF GROWTH HORMONE IN HUMAN PLASMA, The Journal of Clinical Endocrinology & Metabolism, Volume 19, Issue 9, 1 September 1959, Pages 1049–1054, https://doi.org/10.1210/jcem-19-9-1049
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Abstract
A method is described for the concentration of growth hormone (GH) fromhuman plasma. It is based on recovery experiments of human GH from human plasma and the demonstration of GH in plasma from patients with acromegaly. Human GH treated with trichloracetic acid is soluble in absolute ethanol and precipitates from the ethanol extract by pH-fractionation without any loss of biologic activity. A high concentration of GH was found in the plasma of patients with acromegaly but no activity could be detected in the plasma of normal subjects.