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H. H. COLE, H. GOSS, JAMES BODA, STUDIES ON THE STABILITY OF EQUINE GONADOTROPIN IN CRUDE AND IN PURIFIED FORM, The Journal of Clinical Endocrinology & Metabolism, Volume 10, Issue 4, 1 April 1950, Pages 432–436, https://doi.org/10.1210/jcem-10-4-432
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Abstract
IN STUDYING the biologic and chemical properties of equine gonadotropin, a factor of immediate concern is the stability of the hormone under varying conditions. Furthermore, for clinical purposes, it is desirable that the hormone be highly purified. Several reports reviewed by Østergaard (1) and Leathern (2) indicate that allergic response or antigonadotropin formation may occur following the use of certain commercial preparations. Thus, the feasibility of further purification should be explored. Earlier studies (3) have shown that the hormone can be prepared in a highly purified form without excessive loss. The present paper is concerned with the stability of purified extracts as compared to that of crude pregnant mare serum.
These studies were initiated in 1934. For all of the assays on crude pregnant mare serum, the method of Cole, Guilbert, and Goss (4) was used. Some of the purified extracts were tested by this method and some by the more accurate method suggested by Cole and Erway (5). For assay, all powders were weighed on a semimicrobalance and diluted with saline.