-
Views
-
Cite
Cite
FRANCESCO BEGUINOT, DONATELLA TRAMONTANO, CARLO DUILIO, SILVESTRO FORMISANO, LAURA BEGUINOT, PIERLUIGI MATTIOLI, MARIO MANCINI, SALVATORE M. ALOJ, Alteration of Erythrocyte Membrane Lipid Fluidity in Human Obesity, The Journal of Clinical Endocrinology & Metabolism, Volume 60, Issue 6, 1 June 1985, Pages 1226–1230, https://doi.org/10.1210/jcem-60-6-1226
- Share Icon Share
Abstract
The lipophilic probe l,6-diphenyl-l,3,5-hexatriene was incorporated into erythrocyte ghosts of either normal or obese humans, and the polarization of fluorescence was measured between 0 and 40 C. The membrane lipid fluidity, evaluated by fluorescence polarization, was consistently higher in the ghosts from obese subjects. A strong correlation was found between increased l,6-diphenyl-l,3,5-hexatriene fluorescencepolarization and excess body weight. Measurements of cholesterol and phospholipids indicated increased cholesterol and decreased phospholipids in erythrocyte ghosts from obese subjects. These data suggest that alterations in lipid composition in erythrocytes of obese subjects are responsible for abnormal physical properties of plasma membranes, which, in turn, may cause altered enzymatic activities.