Previous studies demonstrated that a low molecular weight peptide fraction (G10-3) from human follicular fluid (FF1) could inhibit steroidogenesis by rat granulosa cells in vitro. In the present study this FF1 fraction was tested upon human granulosa cells. The G10-3 fraction (<1000 mol wt) was obtained by sequential gel filtration on Sephadex G50 and G10 of a steroid-free extract of a pool of human FF1 collected from various-sized follicles at different stages of the menstrual cycle. Human granulosa cells were obtained from large, healthy follicles in the mid- to late follicular phase of eight patients, and cultured for 4–6 days in the absence or presence of G10-3. In all cases G10-3 produced a dose-dependent inhibition of basal progesterone secretion and, when tested, also of FSH-stimulated progesterone secretion. Inhibition of progesterone secretion appeared to be greater in cells obtained from less mature follicles as compared to cells obtained from follicles that were periovulatory. The results suggest the presence of a low molecular weight luteinizing inhibitor in human FF1. (J Clin Endocrinol Metab56: 835, 1983)

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