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Penghao Gu, Yu Zhou, Dengyang Wang, Yu Tian, Yaoqiang Wang, Haijia Su, Optimization of fermentation conditions for enhanced hydrogen production by newly isolated Clostridium butyricum ZYZCB strain, Journal of Applied Microbiology, Volume 136, Issue 5, May 2025, lxaf096, https://doi.org/10.1093/jambio/lxaf096
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Abstract
The foundation of biohydrogen production lies in the utilization of efficient hydrogen-producing strains. This study aims to isolate highly efficient hydrogen-producing strains and enhance hydrogen production performance by optimizing the fermentation process.
A hydrogen-producing bacterial strain was isolated from air and identified by 16S rDNA sequence analysis as belonging to the species Clostridium butyricum, and was named C. butyricum ZYZCB. The results indicate that the ZYZCB strain exhibits notable advantages in terms of rapid growth and a shortened lag phase. ZYZCB can produce hydrogen within a pH range of 5–9 and a temperature range of 25°C–40°C. The utilization of starch, maltose, and sucrose as substrates, resulted in a hydrogen production exceeding 1750 ml l−1, with a maximum hydrogen yield of 2.28 mol H2 mol−1 glucose for ZYZCB, proving the suitability of strain ZYZCB for H2-producing dark fermentation.
The ZYZCB strain exhibits favorable characteristics during fermentation and hydrogen production, showing potential for application in the field of biohydrogen production.