Abstract

Aims

The foundation of biohydrogen production lies in the utilization of efficient hydrogen-producing strains. This study aims to isolate highly efficient hydrogen-producing strains and enhance hydrogen production performance by optimizing the fermentation process.

Methods and results

A hydrogen-producing bacterial strain was isolated from air and identified by 16S rDNA sequence analysis as belonging to the species Clostridium butyricum, and was named C. butyricum ZYZCB. The results indicate that the ZYZCB strain exhibits notable advantages in terms of rapid growth and a shortened lag phase. ZYZCB can produce hydrogen within a pH range of 5–9 and a temperature range of 25°C–40°C. The utilization of starch, maltose, and sucrose as substrates, resulted in a hydrogen production exceeding 1750 ml l−1, with a maximum hydrogen yield of 2.28 mol H2 mol−1 glucose for ZYZCB, proving the suitability of strain ZYZCB for H2-producing dark fermentation.

Conclusions

The ZYZCB strain exhibits favorable characteristics during fermentation and hydrogen production, showing potential for application in the field of biohydrogen production.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://dbpia.nl.go.kr/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
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