Volume 25, 2025

A yeast platform was developed for evaluation of ligands modulating the human free fatty acid 2 receptor activity, in a system devoid of regulatory mechanisms and crosstalk.

The development of standard PCR/ImageJ assay that assessed the telomere length based on the amplified PCR band intensity using telomere primers, equivalent to telomeric sequence availability in the genome.

This study introduces a novel screening approach to identify yeast strains with high pectinolytic activity, optimizing polygalacturonase production for improved applications in wine-making and other pectin-rich industries.

Experimental evolution of Saccharomyceseubayanus Patagonian strains, followed by hybridization allows genetic improvement for beer fermentation.

Aromatic fermented rice milk is modified by a biotechnological process using the flavor yeast Saccharomyces cerevisiae RSO4 strain with good fermenting ability. An integrated omics approach identified specific genes representing genetic variants related to carbohydrate metabolism as well as flavor and aroma production. Innovative rice milk fermentation using novel potentially probiotic yeasts provides value to by-products and plant residues obtained from the agro-food industry, while delivering a high-quality final product with good aroma, umami taste, and health benefits.

This paper provides a comprehensive and up-to-date review on improving the heme supply, enhancing hemoprotein expression, and harmonizing the synthesis of heme and the expression of protein components in yeast.

This retrospective recounts the discovery of drug efflux protein Cdr1, a major determinant of antifungal drug resistance.

This study provides valuable insights into the microbial composition of kombucha, highlighting the stable core microbiota and the dynamics of accessory taxa across fermentation cycles.

The study analyses the genetic and phenotypic diversity of Monosporozyma unispora strains from fermented foods, revealing low nucleotide diversity, clonal reproduction, and adaptation to specific environments such as cheese, kefir, and sourdough.

This study aimed at exploring ethylene glycol metabolism in Saccharomyces cerevisiae and to improve its conversion into glycolic acid by metabolic engineering, uncovering the first reaction as the bottleneck of the pathway.

Cellular and molecular evidence is provided that production-limiting bottlenecks in Komagataellaphaffii cell factories, arising from burdensome heterologous protein secretion, are multifaceted, with respiration as the overarching factor.

Discovery of an acyltranferase from red dragon fruit that enables recombinant production of the red pigment phyllocactin in Saccharomyces cerevisiae and Yarrowia lipolytica.

This minireview highlights advancements in Komagataella phaffii expression systems, focusing on genetic engineering, transcriptional and translational regulation, protein folding and secretion optimization, omics technologies, and high-throughput screening, establishing its potential as a next-generation biotechnological platform.

Saccharomyces cerevisiae is a Crabtree-positive yeast and accumulates ethanol as main product. This review discusses the efforts to create Crabtree-negative stains and the applications to produce chemicals beyond ethanol.

This article covers genetic tools, computational approaches, and metabolic engineering successes in both conventional (Saccharomyces cerevisiae) and non-conventional (Yarrowia lipolytica, Rhodotorula toruloides, and Candida krusei) yeasts.

Allowing an engineered baker’s yeast strain to utilize glycerol via a second metabolic pathway caused strongly increased carbon flux and ethanol formation, suggesting a regulatory activity of the enzyme glycerol kinase.

CRISPR-Cas genome-wide screens enable precise, high-throughput gene editing for industrial applications.

Image-based quantification of Candida albicans germination and filamentation using the open-source visual programming language JIPipe—this enables life scientists to perform.

This article critically reviews the homologous and heterologous expression systems that have been used to study Candida albicans ABC drug efflux pump Cdr1.

In a human fungal pathogen, the putative transcription factor Zcf11 has no known function. Here, it is shown that Zcf11 is a negative regulator of genes involved in meiosis.

The recent advancements in engineering of single carbon (C1) utilization pathways in yeast biotechnology for future C1-based processes are discussed.