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Michael G. Rosenfeld, Itamar B. Abrass, Bieshia Chang, Hormonal Stimulation of α-Amylase Synthesis in Porcine Pancreatic Minces, Endocrinology, Volume 99, Issue 2, 1 August 1976, Pages 611–618, https://doi.org/10.1210/endo-99-2-611
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Abstract
Minces of porcine pancreas maintained a linear rate of protein synthesis for more than 4 h with similar kinetics in the presence or absence of hormones. α-Amylase protein, quantitated by using glycogen precipitation, SDS polyacrylamide gel analysis, and radioimmunoassay, was found to represent 6-7% of the total protein content of the minces. The addition of prostaglandin E1 (10-7m), or a pancreozymin preparation (3-10 Crick-Harper-Raper units/ml) to the incubation media produced within 60 min an increase in α-amylase protein above that stored in the tissue 10- to 20-fold greater than that observed in control minces. The addition of cAMP analogs (10-3m) to the medium produced a more variable stimulation of α-amylase content. Quantitation of the incorporation of radiolabeled amino acid into α-amylase protein with simultaneous determination of the specific activity of the precursor pool suggested that these agents produced at least 3- to 5-fold stimulation of de novo α-amylase synthesis. This stimulation was not the result of decreased rates of α-amylase catabolism. The addition of actinomycin D (20 μg/ml), a concentration inhibiting more than 93% of new RNA synthesis, failed to alter the stimulation of α-amylase synthesis by these agents. Apparent hormonal stimulation of non-α-amylase protein synthesis was also observed.
