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J. H. JEPSON, Concentration of the Soluble Organic Phosphate Pool (2,3-DPG) of Mouse Erythrocytes During the Course of the Estrous Cycle and Pregnancy, Endocrinology, Volume 95, Issue 5, 1 November 1974, Pages 1252–1258, https://doi.org/10.1210/endo-95-5-1252
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The mean concentration of the soluble organic phosphate pool (2,3-DPG) of erythrocytes of female mice was greater than that of males. This pool is predominantly 2,3-DPG and, to a lesser extent ATP. Their concentration in the erythrocyte was shown to increase through the estrous cycle with the lowest concentration observed during proestrus. The concentration did not appear to be inversely related to either the concentration of hemoglobin or the mean cell hemoglobin concentration (MCHC) of the erythrocyte. During pregnancy in the mouse the concentration of the soluble organic phosphate pool in erythrocytes increased between the 9th and 19th day of gestation. In the number of mice studied, this increase did not appear to be inversely related to the MCHC, to the hemoglobin concentration, or to the number of fetuses.
A striking increase of the concentration of the erythrocyte soluble organic phosphate pool occurred following injection of 10 μg of estradiol valerate per day, for 3 days, into normal female mice. At 25 μg per day, for 3 days, no significant effect was evident and the reasons for this are discussed. Testosterone cyclopentyl proprionate induced a striking increase of the concentration of the soluble organic phosphate pool in mouse erythrocytes when compared to diluent treated controls. The effect of 17α-hydroxyprogesterone caproate was equivocal. The data suggest that endocrine changes during estrus, pregnancy or those induced by injection of sex steroids alter, directly or indirectly, the concentration of 2,3-diphosphoglycerate in mouse erythrocytes. (Endocrinology95: 1252, 1974)