We previously reported methods for the preparation of enriched and purified dispersed somatotrophs from the rat adenohypophysis. We now demonstrate that the somatotrophs retain (a) their GH content during the purification proceduresand (b) their responsiveness to dibutyryl cyclic AMP and to a partially purified hypothalamic extract, rich in GHRF (GH-releasing factor) activity. This dispersed somatotroph preparation may provide a novel and useful tool in the study of the mechanisms governing the synthesis and release of GH. (Endocrinology94: 1525, 1974)

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