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WALTER E. STUMPF, Nuclear Concentration of 3H-estradiol in Target Tissues. Dry-Mount Autoradiography of Vagina, Oviduct, Ovary, Testis, Mammary Tumor, Liver and Adrenal, Endocrinology, Volume 85, Issue 1, 1 July 1969, Pages 31–37, https://doi.org/10.1210/endo-85-1-31
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Distribution of radioactivity in target and nontarget tissues has been studied by dry-mount high resolution autoradiography at different time intervals after the injection of 6,7-3H-estradiol-17β in intact immature and castrated mature rats. A nuclear concentration of 3H-estradiol appears to be characteristic for all target tissues studied, that is, in epithelial cells, connective tissue cells and muscle cells of the uterus, vagina and oviduct; in epithelial cells of the induced mammary tumors; in acidophiles, basophiles and chromophobes of the adenohypophysis; and in neurons concentrated in circumscribed areas of the brain. Radioactivity was also concentrated in nuclei of granulosa cells of the ovary and interstitial cells of the testis. In target cells the silver grain concentration over nuclei appeared random and without accumulation at nucleoli. A relatively small amount of radioactivity was also found in the cytoplasm. In nontarget tissues such as liver and adrenal, no nuclear concentration of radioactivity existed. The nuclear concentration of the hormone is interpreted as being suggestive of a genomic effect not only in peripheral target tissues but also in the pituitary and brain, that is, in tissues associated with the feedback control of sexual functions. (Endocrinology85: 31, 1969)