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WALTER E. STUMPF, Subcellular Distribution of 3H-estradiol in Rat Uterus by Quantitative Autoradiography—A Comparison Between 3H-estradiol and 3H-norethynodrel, Endocrinology, Volume 83, Issue 4, 1 October 1968, Pages 777–782, https://doi.org/10.1210/endo-83-4-777
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Abstract
0.09 or 0.63 μg of 6,7-3H-estradiol- 17β or 10 μg of 6,7-3H-norethynodrel was injected in immature rats and the distribution of radioactivity was studied by autoradiography. The autoradiographic technique used is based on low temperature tissue preparation of freeze-dried frozen sections and dry mounting on photographic emulsion, eliminating known sources of translocation artifacts such as liquid fixation, embedding and wet tissue and wet emulsion mounting. Autoradiograms were prepared 15 min and 2 hr after subcutaneous injection. 3H-estradiol was found to be concentrated in nuclei of cells of the epithelium of the lumen and the glands, the substantia propria and the muscularis. Quantification of the subcellular distribution of radioactivity by silver grain counting showed about 68% of 3H-estradiol associated with the nuclear compartment of all the uterine tissues. This value agrees with results in the literature from centrifugal fractionation experiments. Cell nuclei were not uniformly labeled, and a small fraction did not show radioactivity at all under the conditions applied. The silver grain distribution in the cell nuclei per 10 μ2 of nuclear area (average specific concentration) appeared to be similar in the epithelium, substantia propria and muscularis. In contrast to 3H-estradiol, after the injection of 3H-norethynodrel, radioactivity was not found to be concentrated in cell nuclei. (Endocrinology83: 777, 1968)