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L.J. CIZEK, M.R. NOCENTI, S. OPARIL, Sex Difference in Fluid Exchange During Food Deprivation in the Rabbit, Endocrinology, Volume 78, Issue 2, 1 February 1966, Pages 291–296, https://doi.org/10.1210/endo-78-2-291
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Daily measurements of body weight, water and food intakes and urinary Na+, K+, Cl-, osmolarity and volume were made during 7 days of ad lib. feeding, followed by 7 days of food deprivation in normal male, and normal and ovariectomized female rabbits. Male rabbits deprived of food show a marked increase in fluid intake and urine output which is associated with an enhanced Na+ excretion. In contrast, female rabbits display a diminished or even depressed fluid exchange during food withdrawal; the onset of the increased Na+ excretion, although present as in the male, is delayed. Ovariectomy converts the female type of response to that of the male, i.e., a polyuria and polydipsia. Diethylstilbestrol (100 μg/day) or estradiol (100 μg/ day) given to the male on the 3rd and 4th days of food deprivation results in a significantly reduced water intake and urine output and increased urinary osmolar concentration; similar results are obtained in estradioltreated ovariectomized rabbits. Possible mechanisms of this estrogen effect are discussed. (Endocrinology78: 291, 1966)