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SOL L. RABASA, FRIDA BERGMANN, A METHOD TO REDUCE THE INDIVIDUAL VARIATION IN AN ANTIDIURETIC ASSAY, Endocrinology, Volume 60, Issue 5, 1 May 1957, Pages 597–601, https://doi.org/10.1210/endo-60-5-597
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Abstract
ALONG series of methods have been proposed for the assay of the antidiuretic activity of different substances. Some of them have achieved an outstanding sensitivity, as the method of Dicker (1), but it requires a complicated apparatus, which is not at hand in every laboratory. Ginsburg and Heller's procedure (2), though simpler, needs also the previous cannulation of the external jugular vein and a high percentage of animals fails to reach the rate of urine excretion which is needed to begin the assay. Crawford and Pinkham's method (3) is complicated by the determination of the freezing point. The specificity afforded by this technique is valuable in some instances but is unnecessary in standard assays. The present authors have chosen, for practical purposes, the method of Stein, Jinks and Mirsky (4), because the test substance is injected intraperitoneally and the rats need no special previous preparation; its sensitivity being, on the other hand, generally sufficient. The results are sometimes erratic, however, and the need of duplicates or of enlarging the number of animals per group makes this method open to criticism. This experiment is concerned with the search of this source of confusion.
