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E. DICZFALUSY, A.-M. MAGNUSSON, L. NILSSON, A. WESTMAN, A METHOD OF BIOASSAY BASED ON THE DURATION OF ACTION OF ESTROGENS, Endocrinology, Volume 60, Issue 5, 1 May 1957, Pages 581–588, https://doi.org/10.1210/endo-60-5-581
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Abstract
THE increasing therapeutic use of long-acting sex hormone preparations seems to indicate that bioassay methods based on the duration of action of such preparations might be of value. The introduction into therapeutics of poly-estradiol phosphate (P.E.P.), a water soluble high molecular weight polyester of phosphoric acid and estradiol-17β (1, 2) led to the need of a method of comparing the prolonged estrogenic effect of various P.E.P.-preparations. The present paper reports on a method of bioassay based on the duration of vaginal cornification in spayed mice.