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BERNARD KLIMAN, HILTON A. SALHANICK, M. X. ZARROW, THE RESPONSE OF THE PUBIC SYMPHYSIS OF THE MOUSE TO EXTRACTS OF PREGNANT RABBITSERUM AND PREGNANT SOW OVARIES AND ITS APPLICATION AS AN ASSAY METHOD, Endocrinology, Volume 53, Issue 4, 1 October 1953, Pages 391–402, https://doi.org/10.1210/endo-53-4-391
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Abstract
GARDNER reported in 1935 that the pubic symphysis of the mouse separates during pregnancy and that a small amount of separation could be obtained by prolonged treatment of castracted mice with estrogens (Gardner, 1936). Hall and Newton (1946, 1947), however, showed that it was necessary to inject castrated mice with pregnant rabbit serum (PRS) in addition to a preliminary treatment with estrone in order to duplicate the rapid rate of separation observed during pregnancy. Further work by Hall (1948) indicated the necessity of pretreating the mice with estrogen for a period of five to eight days in order to obtain the maximum response to PRS.
Because PRS is an excellent source of relaxin, and since the same type of response, i.e. pelvic relaxation, is obtained in both the mouse and guinea pig, Hall (1948) suggested that the measurement of pubic relaxation in the mouse could be used as an assay for relaxin.