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REPRODUCTIVE PERFORMANCE OF FEMALE MICE TREATED PREPUBERALLY WITH A SINGLE INJECTION OF ESTRADIOL DIPROPIONATE, Endocrinology, Volume 53, Issue 3, 1 September 1953, Pages 342–343, https://doi.org/10.1210/endo-53-3-342
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Abstract
Female rats treated prepuberally with various estrogens have been reported to be deficient in reproductive ability at maturity (Greene and Burrill, 1941; Turner, 1941; Hale, 1944; Hale and Weichert, 1944). Wilson (1943) found that injections of estradiol dipropionate (12 injections totaling 1.2 mg. in 28 days), when begun at one, five or ten days of age, prevented reproduction in female rats. Rats in which treatment was begun at one or five days had small ovaries with small follicles, atretic follicles and no corpora lutea. Those started at 10 days of age had normal follicles but corpora lutea were inconstant. When treatment was initiated after 10 days of age, the animals recovered. Ovaries were normal with regard to follicles and corpora lutea and pregnancy ensued upon mating with normal males.
In this study a single injection of either 1μg. or 10 μg. of estradiol dipropionate2 was tested for it? effect on reproduction.