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Aihua Cai, James F. Hyde, Upregulation of Leptin Receptor Gene Expression in the Anterior Pituitary of Human Growth Hormone-Releasing Hormone Transgenic Mice, Endocrinology, Volume 139, Issue 1, 1 January 1998, Pages 420–423, https://doi.org/10.1210/endo.139.1.5810
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Abstract
The recently cloned leptin receptor (OB-R) is expressed in many tissues, including the anterior pituitary. It is not known whether OB-R gene expression is regulated by pituitary hormones. In the present study, we detected the long isoform of OB-R (OB-RL) in the anterior pituitary of normal mice using RT-PCR, but were unable to detect the short isoform (OB-RS). In human growth hormone-releasing hormone (hGHRH) transgenic mice, we discovered a significant increase in OB-RL mRNA levels in the anterior pituitary as compared to controls, and OB-RS gene expression was detectable. In contrast to the pituitary, there were no significant changes in OB-R gene expression for either isoform in the hypothalamus of hGHRH mice. The dramatic increase in the gene expression of OB-RL in the anterior pituitary of hGHRH transgenic mice was confirmed by RNase protection assay. This is the first study to demonstrate that OB-R gene expression in the anterior pituitary gland is increased by GH and/or GHRH.
