-
Views
-
Cite
Cite
FABIENNE L'HORSET, CHRISTINE PERRET, ARLETTE BREHIER, MONIQUE THOMASSET, 17β-Estradiol Stimulates the Calbindin-D9k (CaBP9k) Gene Expression at the Transcriptional and Posttranscriptional Levels in the Rat Uterus, Endocrinology, Volume 127, Issue 6, 1 December 1990, Pages 2891–2897, https://doi.org/10.1210/endo-127-6-2891
- Share Icon Share
The 9 kilodalton vitamin D-dependent calciumbinding protein (CaBP9k), calbindin-D9k, is expressed in the intestine and uterus of mammals. Rat intestinal CaBP9k is a well documented expression of the steroid hormone like action of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). In contrast exogenous 1,25-(OH)2D3 does not affect the concentration of uterine CaBP9k which is dependent on estrogen. We have analyzed the effect of 17β-estradiol on the regulation of CaBP9k gene expression in the uterus of mature ovariectomized rats. CaBP9k mRNA is undetectable in the uterus of mature ovariectomized rats. A single dose of 17β-estradiol results in a detectable level of Ca- BP9k mRNA at 1 h and a significant increase 3 h after injection. The maximal CaBP9k mRNA level is reached 6 to 12 h post injection. These results show that 17β-estradiol increases Ca- BP9k production by increasing CaBP9k gene transcription. Chronic 17β-estradiol administration results in a plateau of CaBP9k mRNA but in a large increase in CaBP9k concentration. The kinetic response to a single estradiol injection was similar in immature rats. This result shows that no cellular differentiation is required for the control of CaBP9k gene expression by 170-estradiol. The uterine cells of immature rats are already competent to respond optimally to estradiol. There is a single 0.5 kilobase CaBP9k gene transcript in the rat duodenum. In contrast there are two estrogen-inducible CaBP9k mRNA species in the uterus of both the mature ovariectomized and the immature rats. The smallest species corresponds to the duodenal CaBP9k mRNA species, while the larger species is at least 50 nucleotides larger. However, a unique CaBP9k identical to that in the duodenum is expressed in the uterus. (Endocrinology127: 2891–2897,1990)