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ROBERT I. GROVE, KENNETH S. KORACH, Estrogen Stimulation of Phosphatidylinositol Metabolism in Mouse Uterine Tissue, Endocrinology, Volume 121, Issue 3, 1 September 1987, Pages 1083–1088, https://doi.org/10.1210/endo-121-3-1083
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Abstract
The effect of estrogens on incorporation of labeled precursor into inositol-containing phospholipids (PI) from ovariectomized mouse uterus was investigated. The results indicate that diethylstilbestrol (DES) a potent mitogen, stimulated incorporation of myo-[3H]inositol into uterine PI in 1-3 h, with maximal incorporation occurring at 6 h. This activity followed a dose-dependent increase, with a maximal effect at 5 μg/kg- Incorporation of radiolabeled phosphorous into the polyphosphoinositides was also increased in estrogen-stimulated uterine tissue. Studies using a weak uterotropic DES derivative, Z,Zdienestrol, produced an early stimulation of the PI response comparable to DES. This activity was increased by Z,Z-dienestrol, with minimal estrogen receptor occupancy, and did not result in stimulation of DNA synthesis. These findings would suggest that uterine PI stimulation may not occur via an estrogen receptor-mediated mechanism related to tissue proliferation induced by estrogens. (Endocrinology121: 1083–1088,1987)