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MARK H. WHITNALL, SHARON KEY, HAROLD GAINER, VASOPRESSIN-CONTAINING AND VASOPRESSIN-DEFICIENT SUBPOPULATIONS OF CORTICOTROPIN-RELEASING FACTOR AXONS ARE DIFFERENTIALLY AFFECTED BY ADRENALECTOMY, Endocrinology, Volume 120, Issue 5, 1 May 1987, Pages 2180–2182, https://doi.org/10.1210/endo-120-5-2180
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Abstract
CRF-containing parvocellular axons in the external zone of the rat median eminence were classified as vasopressin-containing (CRF+/AVP+) and vasopressln-defIcient (CRF+/AVP-) subpopulations based on post-embedding electron microscopic immunocytochemlcal staining of serial ultrathln sections for CRF, AVP and the other peptides derived from the AVP precursor: AVP-assoclated neurophysin (NP-AVP) and the C-terminal glycopeptlde (GP). In normal animals, the CRF+/AVP+ and CRF+/AVP- subpopulations were approximately equal in terms of detectable axonal swellings. Three to 14 days after adrenalectomy (ADX), the CRF+/AVP+ and CRF+/AVP- subpopulations represented about 95$ and 5%, respectively, of total CRF+ swellings. This change was due to a 90)6 decrease In the absolute number of detectable CRF+/AVP- swellings after ADX, whereas the absolute number of detectable CRF+/AVP+ swellings rose by less than 20%. These changes were completely blocked by administering the g1ucocorticoid agonist dexamethasone throughout the period after ADX. The results suggest that the CRF+/ AVP+ and CRF+/AVP- subpopulations of neurosecretory axons in the external zone of the median eminence respond differently to ADX, Indicating that they are independently regulated by gIucocortIcoids.