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LORRAINE A. FITZPATRICK, MARIA-LUISA BRANDI, GERALD D. AURBACH, Calcium-Controlled Secretion Is Effected through a Guanine Nucleotide Regulatory Protein in Parathyroid Cells, Endocrinology, Volume 119, Issue 6, 1 December 1986, Pages 2700–2703, https://doi.org/10.1210/endo-119-6-2700
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Abstract
Calcium regulates parathyroid secretion through mechanisms yet to be elucidated. We have investigated this phenomenon through use of pertussis toxin which catalyzes ADP-ribosylation and inactivation of a guanine nucleotide regulatory protein, possibly Ni or No. Calcium inhibition of PTH release is blocked in cells treated with pertussis toxin, and there is concomitant ADP-ribosylation of a 40-kilodalton protein. The ionophore A23187 inhibits secretion in toxin-treated as well as in control cells. We conclude that a guanine nucleotide regulatory protein is involved in calcium regulation of PTH secretion at a locus proximal to the intracellular site effecting inhibition of secretion. (Endocrinology119: 2700–2703, 1986)