-
Views
-
Cite
Cite
STEVEN C. MILLER, CYNTHIA D. K. BOTTEMA, PETER A. STATHIS, LASZLO G. TOKÉS, DAVID FELDMAN, Unexpected Presence of Estrogens in Culture Medium Supplements: Subsequent Metabolism by the Yeast Sacchromyces cerevisiae, Endocrinology, Volume 119, Issue 3, 1 September 1986, Pages 1362–1369, https://doi.org/10.1210/endo-119-3-1362
Close - Share Icon Share
Abstract
We have previously shown the presence of 17β-estradiol in extracts of commercially prepared Saccharomyces cerevisiae ss well as the production of estradiol by yeast grown in the laboratory. In our current study, yeast grown in a chemically defined medium synthesized estradiol in only small amounts, (<500 pg/liter). We have analyzed a variety of media commonly used for growing yeast and found that substantial estradiol production (>5 ng/liter) was obtained when yeast were grown in medium supplemented with Bacto-peptone. The peptone was shown to contain significant amounts of estrone, and the results of the experiments establish a precursor-product relationship where estrone from the medium is metabolized to estradiol by S. cerevisiae. Studies with added [3H]estrone demonstrated rapid conversion into [3H]estradiol and a 3H-labeled nonpolar estrogen derivative. The commercially obtained yeast used previously had been grown in a molasses medium. We demonstrate here that the molasses medium contains substantial amounts of estrone and estradiol. We conclude that the conversion of estrone in a culture medium to estradiol in laboratory grown yeast and estrone and estradiol present in the commercially grown yeast medium account for the majority of estradiol found in yeast. (Endocrinology119: 1362–1369, 1986)
