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J. EDWARD PUZAS, RUSSELL T. TURNER, GUY A. HOWARD, DAVID J. BAYLINK, CELLS ISOLATED FROM EMBRYONIC INTESTINE SYNTHESIZE 1,25-DIHYDROXYVITAMIN-D3 AND 24,25-DIHYDROXYVITAMIN-D3 IN CULTURE, Endocrinology, Volume 112, Issue 1, 1 January 1983, Pages 378–380, https://doi.org/10.1210/endo-112-1-378
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Cells isolated from embryonic chick intestine in culture convert 25-hydroxyvitamin-D3 to a number of more polar metabolites. Two of these metabolites have been identified with chemical and chromatographic methods as 24,25-dihydroxyvitamin-D3 (24,25(0H)2D3) and 1,25-dihydroxyvitamin-D3 (1,25(0H)2D3). The enzymes conform to substrate saturation' kinetics. The apparent Km for substrate for the synthesis of 1,25(OH)2D3 and 24,25(OH)2D3 is 70 nM and 29 nM, respectively.