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B. MARTINEAU-DOIZÉ, W. H. LAI, H. WARSHAWSKY, J. J. M. BERGERON, In Vivo Demonstration of Cell Types in Bone That Harbor Epidermal Growth Factor Receptors, Endocrinology, Volume 123, Issue 2, 1 August 1988, Pages 841–858, https://doi.org/10.1210/endo-123-2-841
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The binding and internalization of [125I]iodoepidermal growth factor (EGF) by bone cells of the rat was demonstrated in situ by quantitative radioautography. Specific binding sites were observed on a cell profile enriched in endocytic components, including lysosome-like structures, a rough endoplasmic reticulum-rich cell profile, and a cell profile that histologically resembles an undifferentiated precursor cell. By the criteria of gel filtration and precipitability by trichloroacetic acid, most of the bound [125I]iodo-EGF was considered intact. By morphological criteria none of the cell profiles that bound [125I]iodo-EGF corresponded to fully formed osteoclasts or osteoblasts. The endocytic cell was found in the epiphyseal plate between the invading capillary and the transverse and longitudinal cartilage septa as well as near osteoclasts in the zone of mixed spicules. The rough endoplasmic reticulum-rich cell was present in vacated chondrocyte lacunae of the epiphyseal plate close to the metaphysis, and the poorly differentiated cell was observed between the mixed spicules of the metaphysis. Similar cell types were also found in the alveolar bone surrounding the incisors.
These cells may be the origin of established bone cell lines that harbor high concentrations of EGF receptors and may also be responsible for the humoral hypercalcemia in response to the reported actions of injected EGF or transforming growth factorα as well as that of malignancy. (Endocrinology123: 841–858, 1988)
