Abstract

A chiral pyrromethene–BF2 complex dye, showing absorption (λmax) and emission maxima (λem) at 499 and 518 nm in acetonitrile, respectively, was synthesized. Racemic mexiletine smoothly reacted with this reagent in the presence of EDC to preferentially produce the corresponding diastereomeric amides, which were nicely separated by a non-chiral reverse phase column. The detection limit (S/N > 3) of mexiletine was ca. 0.3 pmol.

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