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Tomokiyo Doi, Takatoshi Kinoshita, Yoshiharu Tsujita, Hiroaki Yoshimizu, Control of Molecular Orientation of α-Helix in the Monolayer at Air–Water Interface, Bulletin of the Chemical Society of Japan, Volume 74, Issue 3, March 2001, Pages 421–425, https://doi.org/10.1246/bcsj.74.421
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Abstract
Amphiphilic block copolymers of poly(L-leucine-co-ethylene glycol) (PLeum-PEGn) having three different compositions, m : n = 80 : 23, 57 : 46, and 66 : 114, were prepared and spread at an air-water interface to form a stable monolayer. The α-helical conformation of PLeu segment in the monolayers was confirmed by FT-IR and circular dichroism measurements. The molecular orientation of PLeu helix rods in the monolayer was estimated by surface pressure-area isotherm and FT-IR reflection absorption spectroscopy measurements. As a result, we confirmed that α-helix rod of PLeu segment oriented slightly normal to the membrane plane. Furthermore, it was indicated that each α-helical rod in the PLeu57–PEG46 monolayer is effectively oriented normal to the interface compared to that in the PLeu80–PEG23 and PLeu66–PEG114 monolayers.