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Yasuhiro SHIGA, Makiko MAKI, Toshihiro OHTA, Shin-ichi TOKISHITA, Akou OKAMOTO, Norihiro TSUKAGOSHI, Shigezo UDAKA, Atsushi KONISHI, Yuko KODAMA, Daisuke EJIMA, Hiroshi MATSUI, Hideo YAMAGATA, Efficient Production of N-terminally Truncated Biologically Active Human Interleukin-6 by Bacillus brevis, Bioscience, Biotechnology, and Biochemistry, Volume 64, Issue 3, 1 January 2000, Pages 665–669, https://doi.org/10.1271/bbb.64.665
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Abstract
cDNAs encoding human interleukin 6 (hIL-6) and its variants lacking the N-terminal Pro and Pro-Val-Pro-Pro, respectively, were expressed in Bacillus brevis by using the signal peptide fusion approach. The presence of Pro at the N-terminus of the mature protein hindered the action of the Bacillus brevis signal peptidase. hIL-6 lacking the N-terminal Pro-Val-Pro-Pro was most efficiently secreted in a biologically active form and accumulated in the culture medium to a level of 200 mg per liter, which is the highest level reported for the bacterial secretion of hIL-6.