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Tokuki SAKIYAMA, Hideto TAKAMI, Naotake OGASAWARA, Satoru KUHARA, Tokio KOZUKI, Kosuke DOGA, Akira OHYAMA, Koki HORIKOSHI, An Automated System for Genome Analysis to Support Microbial Whole-genome Shotgun Sequencing, Bioscience, Biotechnology, and Biochemistry, Volume 64, Issue 3, 1 January 2000, Pages 670–673, https://doi.org/10.1271/bbb.64.670
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Abstract
We developed a semi-automated genome analysis system called GAMBLER in order to support the current whole-genome sequencing project focusing on alkaliphilic Bacillus halodurans C-125. GAMBLER was designed to reduce the human intervention required and to reduce the complications in annotating thousands of ORFs in the microbial genome. GAMBLER automates three major routines: analyzing assembly results provided by genome assembler software, assigning ORFs, and homology searching. GAMBLER is equipped with an interface for convenience of annotation. All processes and options are manipulatable through a WWW browser that enables scientists to share their genome analysis results without choosing computer platforms.