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Robert Hansen, Shari deSilva, G.Thomas Strickland, Antisporozoite antibodies in mice immunized with irradiation-attenuated Plasmodium berghei sporozoites, Transactions of The Royal Society of Tropical Medicine and Hygiene, Volume 73, Issue 5, 1979, Pages 574–578, https://doi.org/10.1016/0035-9203(79)90056-7
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Abstract
Sera from NMRI/NIH mice were tested for the presence of IgM and IgG anti-sporozoite antibodies using the indirect fluorescent antibody test (IFAT). Both IgM and IgG antibody titres were related to the number of immunizations with irradiation-attenuated Plasmodium berghei sporozoites, and protection from challenge with subsequent non-attenuated sporozoites correlated with the pre-challenge antibody titre. Sera taken five days following challenge showed marked reductions in antibody titres, except for the group receiving the maximum (four) immunizations. Groups immunized with frozen sporozoites or mosquito tissue antigen developed neither antibodies to sporozoites nor protective immunity; nor did animals infected with parasitized blood. However, sera from mice immunized four times with attenuated sporozoites demonstrated IFA titres to blood-stage antigens.
The results show that both IgM and IgG anti-sporozoite antibodies could be detected in mice immunized with attenuated-sporozoites by IFAT, and that the antibody titres correlated with protective immunity. Cross reaction with blood-stage antigens occurred, but the test should still prove useful.
- magnetic resonance imaging
- antigens
- cross reactions
- culicidae
- fluorescent antibody technique
- fluorescent antibody technique, indirect
- immunity
- immunization
- united states national institutes of health
- plasmodium berghei
- immunoglobulin g
- immunoglobulin m
- antibodies
- mice
- igg antibody
- serum
- attenuation
- antibody titer
- sporozoites
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