... ). As discussed above, the accumulation peak at the end of the gene reflects a reduction in the Pol II movement linked to the termination of transcription and polyadenylation ( Porrua et al., 2016 ; Proudfoot, 2016 ). The meaning of the Pol II accumulation near the TSS, on the other hand, is still...

... in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence, enforcing efficient transcriptional termination is difficult. The termination hairpin found natively in the T7 genome is adapted to be inefficient...

... initiation factor 2 (IF2), that each lead to the deletion of the IF2α isoform, while the IF2β and IF2β′ isoforms remain intact ( Sacerdot et al. 1992 ). One isolate had a truncated nusA gene, encoding termination–antitermination factor NusA, and one isolate had a mutation in the regulatory region...

... resequencing, and deep sequencing. Therefore, the second-generation sequencing technology, characterized by its high speed, high throughput, and lower cost, appeared on the scene [3–5] . Reversible termination sequencing technology is a sequencing-by-synthesis approach [3–5] that infers the sequence...

... in the bacterial genome. The stx genes are expressed from p_R′ as a late protein, and anti-terminator activity from the Q protein is necessary for read through of the late terminator t_R′ and activation of p_R′. We investigated...

...–902 (2002). 16) Hargrove JL and Schmidt FH, FASEB J., 3 , 2360–2370 (1989). Biosci. Biotechnol. Biochem., 75 (11), 2234 2236, 2011 Note TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces cerevisiae Expression System Mamoru YAMANISHI, Satoshi KATAHIRA, and Takashi...

... of a transcriptional terminator that supports increased levels of expression. The terminators of several Arabidopsis genes were examined in transfected Arabidopsis T87 protoplasts. The heat shock protein 18.2 (HSP) terminator was the most effective in supporting increased levels of expression...

... and in different tissues indicating differences in regulation. To analyse and compare regulatory sequences of Musa rbcS genes, promoter and terminator regions were cloned for three Musa rbcS genes. Transient transformation assays using promoter–reporter–terminator constructs in maize, wheat...

... industry technology innovation welfare terminator gene O340 Q110 Q160 Q180 The ongoing debate concerning the patenting of plant genes and biotechnological advances motivates our interest in a model that links the protection of intellectual property in the private seed industry with welfare changes...

... protein A RTE    rabbit thymus extract SjS    Sjögren's syndrome SLE    systemic lupus erythematosus Top I    topoisomerase I TTF-1    transcription terminator factor 1 The protein profile of the purified PCNA was analyzed by SDS–PAGE ( 2 ). The slab gel consisted of 12.5% acrylamide and 0.1% SDS...

... in genome. Overlapping of terminator and promoter of two adjacent genes have been reported in E. coli[ 14 , 15 ] and yeast [ 16 ], but no such report in cyanobacteria. We previously isolated a strong promoter-active fragment (designated E10), which responds to light intensity...

.... sonnei and Salmonella groups of organisms. Possible implications of these observations, in connection with the phenotypic variability seen in β-glucoside utilization amongst these members, are discussed. β-Glucoside Cryptic gene Anti-terminator Enterobacteriaceae Members...

...R.G Wake 2 Sequences of the terminus region terminators of B. subtilis 168 and W23. Each terminator is made up of two overlapping sites (A and B). The B site binds RTP with high affinity and is proximal to the fork to be arrested; the A site binds RTP with low affinity. Fully conserved...

... Amidohydrolase Gene mapping Bi-directional terminator opuA operon Promoter References [1] Palm G.J. Röhm K.-H. ( 1995 ) Aminoacylase I from porcine kidney: identification and characterization of two major protein domains . J. Prot. Chem. 14 , 233 – 240 . [2...

... reserved 1996 Abstract Two plasmid cloning vectors (pULMJ55 and pULMJ95) were constructed for Brevibacterium lactofermentum using the origin of replication of the endogenous plasmid pBL1. Plasmid pULMJ55 is a replacement vector with transcriptional terminators from the B. lactofermentum...

... ear (two/head) was used on all adult cattle. Terminator (diazinon) tags were placed in a herd of 49 cows and two bulls on 10 May. Saber (lambdacyhalothrin pyrethroid) tags were placed in a herd of 81 cows and two bulls on 3 May. Tomahawk (pirimiphos methyl) tags were placed in a herd of 52 cows...

...Nicolas Bardonnet; Carlos Blanco Terminator Promoter-probe vector uidA Gene β-Glucuronidase Corynebacteria References [1] Nakayama K. ( 1985 ) In: Comprehensive Biotechnology ( Moo-Young M. , (Ed.), pp. 607 – 620 . Pergamon Press , Oxford . [2] Yeh...

... bull on 4 May and remained for 20 wk. Terminator tags were placed in a herd of 52 cows and 2 bulls on 17 Apr and remained for 20 wk. Saber tags were placed in a herd of 49 cows and one bull on 7 Jul and remained for 12 wk. Untreated herds of 13-23 cows were located on each farm. Herds were mixed breeds...

...′-terminus can be produced even when a circular DNA is used as a template, this kind of construct has a potential to release an ‘active ribozyme’ tailored to destroy a target gene (RNA) in vivo. Moreover, the second ribozyme in this construct can be utilized as a universal pseudo-terminator...

...J. D. Webb; S, M. Presley; F, W. Knapp © 1989 Entomological Society of America. 1989 Abstract Two experimental organophosphate ear tags (YT-802B1 and YT-607C3) were compared with 4 commercially available ear tags (Optimizer, Maxcon Super, Tomahawk, and Terminator) for efficacy against horn flies...