... molecular mechanisms in response to copper stress. In Saccharomyces cerevisiae, transcription factor Ace1 is activated and regulates CUP1, CRS5, and SOD1 in response to copper stress. Hyperoside (HYP) is mainly used in clinical treatment and healthcare products due to its...

... the toxicity of misfolded superoxide dismutase 1 (SOD1) in familial amyotrophic lateral sclerosis via blocking SOD1 expression in the CNS are indiscriminative for misfolded and intact proteins, and as such, entail a risk of depriving CNS cells of their essential antioxidant potential. As an alternative...

... of erythrocyte lysates were performed as previously described. ²⁵ Immunoblot analysis of soluble and detergent-insoluble fractions of SOD1 in patient skin biopsy-derived fibroblast cultures was carried out essentially as described before. ²⁶ We used inhouse produced polyclonal rabbit anti...

..., 5–10% of ALS cases are familial (fALS), caused by specific gene mutations. Mutations in the gene coding for Cu/Zn superoxide dismutase type-1 (SOD1) are responsible for roughly 20% of fALS cases. ⁵ The G93A mutation in SOD1 is one of the best studied fALS mutations and the first...

..., and this effect was related to increased Sod2 activity upregulated by resorcinol treatment. The gene expression assay for antioxidant proteins genes sod1 and sod2 was described in the Electronic supplementary information (ESI)†. Gene-specific primers declared in Table S1 (Supporting Information†) were...

... haemoglobin. Then 10 μl of each sample preparation were loaded on Any kD Stain free Criterion TGX precast gels (Bio-Rad Laboratories). Gels were activated and protein content imaged using a criterion imaging apparatus, before blotting on nitrocellulose filters (Bio-Rad). Primary rabbit anti-human SOD1...

... DDIT4 METABOLISM MG 63 MITOCHONDRIA OSTEOBLAST OSTEOSARCOMA REDD1 ROS SOD SOD1 SOD2 STRESS TUMOR UNFOLDED PROTEIN RESPONSE VDR VITAMIN D VITAMIN D DEFICIENCY VITAMIN D RECEPTOR American Cancer Society IRG 17 183 16 National Cancer Institute 1R01CA215973 01 Miller School...

... following endosymbiotic eukaryogenesis. metallochaperone eukaryogenesis oxidative stress protein interface, SOD1 Motor Neurone Disease Association 10.13039/501100000406 Wright/Oct18/969-799 Superoxide dismutases and reductases are deployed almost ubiquitously throughout nature to prevent damage...

... Kirstein; Wim Robberecht; Frederic Rousseau; Joost Schymkowitz To whom correspondence should be addressed. E-mails: [email protected] ; [email protected] ALS cytotoxicity HSP70 SOD1 Filip Claes and Stanislav Rudyak contributed equally to this work 26 3 2020 31 3...

... < 0.05); therefore, ANOVA analysis of variance with Tukey post-hoc test were performed. Data for SOD1 activity and SOD1 expression were not significantly different from those of normal distribution (P < 0.05); therefore, ANOVA analysis of variance with Tukey post hoc test was used. All...

... to combat ROS, including superoxide dismutases (SODs), catalases, peroxidases and glutathione peroxidases (Manfredini et al. 26 ). The superoxide anion, a highly reactive ROS, can be detoxified by two SOD, Sod1 (Cu, Zn‐containing enzyme) and Sod2 (manganese‐containing enzyme). Sod2 is located...

... to the influential regulation of sod1 and tp53 genes clarified by pathway analysis with reference to experimental and computational results. The study suggested that cytotoxicity of biologically synthesized silver nanoparticles from bacteria depends on strain specificity with significant difference in use of Gram...

... or n108) were ordered from Bloomington Drosophila Stock Center (BDSC) (stock numbers 24492 and 24490, respectively). The names of the dSOD mutations are based on the human SOD1 amino acid numbering system throughout this work. The dsod^X-16 line was a kind gift from W. C. Orr...

..., and diatoms. We embryonically exposed zebrafish, Danio rerio, (transgenically overexpressing a FALS-causing SOD1-G93R mutation) to BMAA to investigate early motor neuron outgrowth in larvae and endurance and fatigability in 5-month adults. SOD1-G93R zebrafish showed decreased embryonic nerve length...

... in the genes coding for SOD1, TARDBP and FUS being the most frequent. Although familial ALS comprises ∼10–15% of all cases, the majority of ALS is considered sporadic without a known family history of ALS. However, in ∼10 % of sporadic ALS cases mutations in known ALS genes...

...-resistance stability by plate streaking and also for identification of genome DNA by PCR as described by Wang et al. [ 41 ]. YADH-2 and SOD enzyme activity of the 50th generation strains were also detected. Copper-and-zinc-containing superoxide dismutase gene, SOD1, was amplified from the genomic...

.... For Permissions, please email: [email protected] 2013 amyotrophic lateral sclerosis genetic background immune system motor neurons SOD1 Our understanding of disease pathogenesis has increased by the study of models of SOD1-related ALS and it is clear that multiple mechanisms contribute to motor...

... of amyotrophic lateral sclerosis (ALS)-linked mutant Cu/Zn superoxide dismutase (SOD1). Cross reactivity might be caused by abnormal exposure of an epitope in the inner hydrophobic region of SOD1 that shares structural homology with the P2X4-immunizing peptide. Here, we raised antibodies against the human SOD1...

... ). Some fraying of the last one or two residues on each end was seen in other strands, but no other widespread secondary structure loss was observed. ALS misfolding molecular dynamics SOD1 unfolding Cu/Zn superoxide dismutase (SOD1) catalyzes the dismutation of superoxide, a byproduct of cellular...

...:https://dbpia.nl.go.kr/journals/pages/open_access/funder_policies/chorus/standard_publication_model ) Abstract Amyotrophic lateral sclerosis (ALS) generally is a late-onset neurodegenerative disease. Mutations in the Cu/Zn superoxide dismutase 1 (SOD1) gene account for approximately 20% of familial ALS and 2% of all...