...Marine Joly; Cécile Conte; Charles Cazanave; Vincent Le Moing; Pierre Tattevin; Pierre Delobel; Agnès Sommet; Guillaume Martin-Blondel Progressive multifocal leukoencephalopathy (PML) is a rare demyelinating disease of the CNS that occurs after reactivation of the JC virus (JCV...

... after nivolumab salvage therapy in a progressive multifocal leukoencephalopathy patient. Analyses of brain biopsy suggest that PD-L1+ macrophages could be the T-cells partnership in immune exhaustion and virus escape. immune checkpoint inhibitor JC virus nivolumab PD-1 progressive multifocal...

... incidence of PML among patients with diseases unassociated with PML. Safety screening protocols for John Cunningham virus and PML are important to prevent new PML cases. HIV JC virus monoclonal antibodies progressive multifocal leukoencephalopathy rituximab This is an observational study of PML cases...

... in plasma might complement progressive multifocal leukoencephalopathy (PML) diagnosis, especially when cerebrospinal fluid (CSF) is unavailable or JCV-DNA not detectable in CSF. In addition, JCV-DNA plasma levels could provide a marker of PML progression. progressive multifocal leukoencephalopathy JC virus...

... (IHC) and immunofluorescence (IF) staining assays were performed as previously described ( 10 , 11 , 23 , 24 ). ISH was performed with full length JCV DNA probe (JC Virus BIO-PROBE®, ENZ-40847, Enzo Diagnostics, Farmingdale, NY). In multiple IHC and IF stainings, primary antibodies from...

... to the cortical GM. In addition, 127 blocks from 94 HIV-positive and -negative patients without JCV-related diseases were used as controls ( Table 1 ). TABLE 1 JC Virus–Related Diseases and Control Patient Samples Copyright © 2015 by the American Association of Neuropathologists, Inc. 2015 Abstract The human...

... (JCV) capsid protein VP1, and a Toll-like receptor 7 agonist used as adjuvant, was well tolerated, and showed a very favorable safety profile and unexpected efficacy that warrant further investigation. progressive multifocal leukoencephalopathy JC virus IL-7 VP1 Progressive multifocal...

... ]. In this study, we report the case of a woman diagnosed with PML after allogenic bone marrow transplantation for secondary myelofibrosis, in whom single-agent rhIL-7 therapy was associated with an improvement in anti-JCV T-cell responses, JC virus clearance from cerebrospinal fluid (CSF), and a dramatic clinical...

... functional impairment under natalizumab therapy. cerebrospinal fluid JC virus memory T cells natalizumab Biogen Idec GmbH and the Else Kröner-Fresenius Stiftung 2011_A123 Besides mature single-positive (SP) CD4⁺ or CD8⁺ lymphocytes, T cells with a CD4⁺ CD8...

...Yukiko Shishido-Hara; Takuya Yazawa; Motoo Nagane; Kayoko Higuchi; Shiho Abe-Suzuki; Morito Kurata; Masanobu Kitagawa; Hiroshi Kamma; Toshiki Uchihara To measure the nuclear area of cells containing punctate signals for either the PML protein or JC virus VP2/VP3, we obtained images using a digital...

... areas ( Fig. 2B , D, F) and were often in close contact with JCV-infected cells ( Fig. 2D , F). Immune reconstitution inflammatory syndrome JC virus Multiple sclerosis Natalizumab Progressive multifocal leukoencephalopathy Progressive multifocal leukoencephalopathy (PML) is a fulminant inflammatory...

...; n = 18). These findings highlight the importance of latent JVC brain infection to the pathogenesis of PML. JC virus progressive multifocal leukoencephalopathy HIV/AIDS immunosuppression real time PCR Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central...

... received immunosuppression with basiliximab, tacrolimus, mycophenolate mofetil and prednisolone. JC virus was detected in the plasma 5 months after transplantation ( Tables 1 and 2 ; Figure 1 ). As this was the first case of JC virus detection in a renal allograft recipient in our centre, he underwent...

... suggests a restrictive infection in neurons. These results indicate that JCV infection of cerebral cortical neurons is a previously underappreciated component of PML pathogenesis. Cortical neurons Demyelination JC virus Progressive multifocal leukoencephalopathy JC virus (JCV) is a ubiquitous human...

... disease states. Although uncommon, among the infections patients with ICL have presented with, JC virus (JCV) infection is particularly worrisome, as the rapid progression of disease is debilitating. JCV, a human polyoma virus, causes infection of oligodendrocytes in the white matter of the brain (Figure...

... The Author 2010. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: [email protected] 2010 JC virus immunodeficiency cidofovir After a discussion of potential options and a review...

... nonspecific staining. JCV Probe 1: Biotin-labeled JC virus Probe (Enzo Diagnosis, Farmingdale, NY). Probe 2: 5′ bCTT TTT AGG TGG GGT AGA GTG TTG GGA GGA TCC TGTT GTT TTC A 3′ (T-antigen, nucleotide position 4283–4320, MAD-1 strain) [ 13 ]. Probe 3: 5′ bGAT GAA TGT GCA CTC TAA TGG...

... in immunosuppressed individuals. Cerebellum Granule cell neurons HIV JC virus Progressive multifocal leukoencephalopathy The polyomavirus JC (JCV) infects 86% of healthy adults without causing any disease ( 1 ); it remains quiescent in the kidneys and in lymphoid organs ( 2, 3 ). In immunosuppressed individuals...

... components of PML-NBs include PML protein and the nuclear body Sp100 protein. Promyelocytic leukemia protein is a tumor suppressor ( 12-17 ), and Sp100 influences transcription and chromatin dynamics ( 18, 19 ). Using COS-7 cells, we found that the JC virus capsid proteins accumulate and are efficiently...

... nephropathy: a (re-)emerging 12. Baksh FK, Finkelstein SD, Swalsky PA, et al. Molecular complication in renal transplantation. Am J Transplant. genotyping of BK and JC viruses in human polyomavirus- 2002;2:25-30. associated interstitial...