... breaks. Our study thus provides experimental evidence that chromatin replication across ^meG induces futile MMR cycling that is assisted by Smarcad1. Graphical Abstract Graphical Abstract DNA replication mismatch repair, O⁶-methylguanine Smarcad1, Xenopus egg...

... for identifying IZs in late S phase. Graphical Abstract Graphical Abstract DNA replication mouse embryonic stem cells polymerase-usage sequencing replication initiation zones replication timing Sasakawa Scientific Research JPMJPR18K7 JPMJFR204X JSPS KAKENHI JP16K07382 JP17H06477 JPMJPR18K8...

..., and compare multiple datasets more conveniently and comprehensively [ 22 , 23 ]. Finally, we determined reference replication origins (rORIs) for five species with relatively conservative origins in large datasets. In eukaryotes, errors arising from the DNA replication process can easily lead to a series...

...Krystian Łazowski; Roger Woodgate; Iwona J Fijalkowska Corresponding author. Laboratory of DNA Replication and Genome Stability, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland. E-mail: [email protected] Corresponding author. klazowski...

... APOBEC3A and APOBEC3B. APOBEC3C produces DNA strand-coordinated mutation clusters, and APOBEC3C mutations are enriched near the transcription start sites of active genes. Surprisingly, APOBEC3C lacks the bias for the lagging strand of DNA replication that is seen for APOBEC3A and APOBEC3B. The unique...

... Transcriptomics and shotgun proteomics depict PTEN signaling, DNA replication, and DNA repair pathways as significant differentiators between sensitive and resistant models. Vulnerability to MLN4924, a NAE inhibitor, is associated with elevated S-phase populations, DNA re-replication, and DNA damage. In a panel...

... and Pol ε, or in the 5′ manner by FEN1 and EXO1. In all these excision pathways, eukaryotic cells have evolved to avoid formation of the active 3′ flap presumably because it can cause genome rearrangements and instability through strand invasion. Precise resolution of stalled or damaged DNA replication...

... carcinoma miR-1972 GZMH cell cycle DNA replication proliferation Abstract Aim To understand the regulatory roles of miR-1972 and GZMH in hepatocellular carcinoma (HCC) and explore their potential as therapeutic biomarkers. Methods In vitro verification of the regulation of malignant...

... within gene bodies. While the passage of RNA polymerase II is a well-characterized chromatin-disrupting force, numerous factors, including histone chaperones, normally stabilize chromatin on transcribed genes, thereby repressing cryptic transcription. DNA replication, which employs a partially...

... variability in the human germline because of their high mutation rates, which have long been attributed largely to polymerase slippage during DNA replication. This model suggests that STR mutation rates should scale linearly with a father's age, as progenitor cells continually divide after puberty...

... et al. 2020 ). Eukaryotic primase complexes synthesize short RNA primers required for initiating lagging strand DNA replication and also contribute to DNA repair and possibly transcription ( Guilliam et al. 2015 ; Yoon et al. 2018 ). Abnormal primase function causes stalled...

... might be a major cause of DNA replication stress. Institute of Cancer Research (ICR) mouse embryos at 14.5 days of gestation were dissected, and the bodies, after removal of the heads, were finely chopped using scissors. The tissue pieces were then cultured in a T-25 (25 cm²) culture flask...

... to lineage trace adult MuSC-derived myonuclei via GFP labeling. We show that during the early response to mechanically induced growth, a significant proportion of GFP+ MuSC-derived myonuclei differentiate and fuse without having undergone DNA replication. Animal procedures were conducted in accordance...

... that DNA replication, cell cycle, and DNA repair were the most significantly affected pathways. We confirmed the concentration-dependent changes in the expression of key genes in these pathways using real-time PCR. mRNA sequencing showed upregulation of a group of genes tightly associated...

... section through the basal body and kDNA network of T. brucei. In this orientation, the kDNA disc is cut orthogonal to its surface. BB, basal body; F, flagellum; TAC, tripartite attachment complex; and kDNA, mitochondrial genome. Abstract Mitochondrial DNA replication is an essential process...

... undergoes extensive processing throughout the cell cycle. DNA undergoes rapid unwinding during S-phase to allow DNA replication. The sister chromatids are tethered to allow chromosome biorientation at the metaphase plate, thus ensuring equatorial chromosome division. Besides this, the DNA is heavily...

... mutation (to ensure it did not produce a fitness effect). Colony counts and analysis are included in Supplementary File 2 . Abstract Collisions between DNA replication complexes (replisomes) and impediments such as damaged DNA or proteins tightly bound to the chromosome lead to premature dissociation...

... in the initiation of DNA replication, which should be considered in the prediction of oriCs. Most of bacterial oriCs contain DnaA box clusters that are recognized and bound by DnaA proteins. Therefore, the DnaA box cluster is considered as an important characteristic for predicting oriCs...

...-conventional interaction with its binding partners. Our findings will provide insight into the development of new therapies for C. difficile infection. Graphical Abstract Graphical Abstract Clostridioides difficile crystal structure DNA replication molecular interaction sliding DNA clamp...

... SAR studies have given pointers as to the mode of action of 1-(S,R_p). Work using non-phosphorylatable analogues and thymidine kinase–deficient cell lines ²³ suggests that although inhibition of DNA replication is involved, substrate phosphorylation...