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Eve M. D. Smith, Angela Midgley, Louise Watson, Caroline Jones, Clarissa Pilkington, Stephen D. Marks, Kjell Tullus, Michael W. Beresford, 331. Relative Expression of Kidney-Associated Proteins in Lupus Nephritis: Identifying Future Urinary Biomarker Targets for Disease Monitoring, Rheumatology, Volume 53, Issue suppl_1, April 2014, Pages i183–i184, https://doi.org/10.1093/rheumatology/keu128.011
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Background: Up to 80% of children with juvenile-onset SLE (JSLE) develop lupus nephritis (LN). Improved methods of detecting LN onset, severity, flares and treatment response are required to prevent irreversible renal damage. Urinary biomarkers for LN including monocyte chemoattractant protein-1 (MCP-1), alpha-1-acid glycoprotein (AGP) and neutrophil gelatinase associated lipocalin (uNGAL) have previously been a focus of investigation within the UK JSLE Cohort study. The aim of this preliminary study was to identify novel urinary protein biomarker targets to facilitate the development of a point of care testing device, which includes a panel of urinary biomarkers.
Methods: Using a human kidney biomarker proteome profiler array (R&D systems Ltd) the relative expression levels of 38 kidney-associated urinary proteins was analysed. Samples from JSLE patients (n = 4) participating in the UK JSLE Cohort study with active LN (British Isles Lupus Assessment Group (BILAG) score of A in renal domain) were compared with age and sex matched healthy controls (HC, n = 2), and renal inflammatory control patients (Henoch-Schonlein purpura HSP, n = 2, displaying active and inactive disease respectively). Informed parental/patient consent/assent was obtained and the study had full ethical approval.
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