Nutri-microbiome epidemiology, an emerging field to disentangle the interplay between nutrition and microbiome for human health

Abstract

Diet and nutrition have a substantial impact on the human microbiome, and interact with the microbiome, especially gut microbiome, to modulate various diseases and health status. Microbiome research has also guided the nutrition field to a more integrative direction, becoming an essential component of the rising area of precision nutrition. In this review, we provide a broad insight into the interplay among diet, nutrition, microbiome, and microbial metabolites for their roles in the human health. Among the microbiome epidemiological studies regarding the associations of diet and nutrition with microbiome and its derived metabolites, we summarize those most reliable findings and highlight evidence for the relationships between diet and disease-associated microbiome and its functional readout. Then, the latest advances of the microbiome-based precision nutrition research and multidisciplinary integration are described. Finally, we discuss several outstanding challenges and opportunities in the field of nutri-microbiome epidemiology.

Introduction

Human microbiota inhabit across various anatomical body sites such as the skin, oral mucosa, saliva, gastrointestinal tract, respiratory tract, urogenital tract, and the mammary gland (Sender et al., 2016), with the gastrointestinal tract being the most heavily studied body site for human microbiome research. Trillions of microbiota inhabit in the human gastrointestinal tract, which are continuously perturbed by daily dietary intake (Sender et al., 2016; Johnson et al., 2019). The complex interaction between diet, nutrition, and the gut microbiota plays an essential role in modulating the host health (Valdes et al., 2018a; Kolodziejczyk et al., 2019). For example, gut microbiota could metabolize dietary nutrients into functional metabolites, such as disease-causing metabolite trimethylamine N-oxide (TMAO), which was associated with higher risk of the cardiovascular diseases (Senthong et al., 2016; Li et al., 2017; Yu et al., 2019; Lee et al., 2021; Wei et al., 2022), or disease protective metabolite short-chain fatty acids (SCFA) that stimulate the secretion of glucagon-like peptide-1 and regulate glucose metabolism (He et al., 2020).

The advent of high-throughput DNA sequencing technologies (such as 16S, ITS, and metagenomics sequencing) enables profiling of the microbial communities, which facilitates the integration of microbiome big-data with large-scale epidemiological studies or consortium, such as the American Gut Project (McDonald et al., 2018), Dutch LifeLines-DEEP study (Tigchelaar et al., 2015), FINRISK study (Borodulin et al., 2018), and Westlake Gut Project (Gou et al., 2022). Moreover, it is widely recognized about the profound variation in the host response to the identical diet or meal (Zeevi et al., 2015; Berry et al., 2020; Ma et al., 2021), which stimulates numerous research to explore the potential mechanism, with gut microbiome becoming the spotlight of research in the field recently (Zeevi et al., 2015; Korem et al., 2017; Johnson et al., 2019; Mendes-Soares et al., 2019; Berry et al., 2020; Asnicar et al., 2021; Suez et al., 2022). Therefore, microbiome (especially gut microbiome)-based precision nutrition research is becoming one of the frontiers in the field of nutri-microbiome epidemiology.

In this review, we will first summarize the findings of microbiome epidemiological studies that investigate the associations of microbiome and microbial metabolites with diet, nutrition, and human diseases. Then, we will review the current progress of the microbiome-based precision nutrition research. Finally, current limitations, challenges, and perspectives of the nutri-microbiome epidemiology field are presented.

Role of diet and nutrition in shaping the gut microbiome

Host diet is key for the symbiotic role of the gut microbiota. On one hand, the gut microbiota depends on the host intake of nutrients for their own survival, on the other, many gut microbes directly participate in the digestive process, producing a variety of nutrients involved in the host metabolism and biology process (Valdes et al., 2018b; Kolodziejczyk et al., 2019). Data from human cohorts and clinical trials are accumulating recently, showing how specific food types (including food groups and nutrients) and dietary patterns, influence the gut microbiome and subsequently host health (Fig. 1 and Table 1).

Food types

Consumptions of vegetables and fruits were associated with higher abundance of SCFA producers. For example, fruit and vegetable intakes were both positively associated with Coprococcus species, Faecalibacterium prausnitzii, Roseburia hominis, and Firmicutes bacterium CAG:95 across several studies (Liu et al., 2019; Jiang et al., 2020; Asnicar et al., 2021; Breuninger et al., 2021). Higher proportions of SCFA-producing bacteria have beneficial effects on the human host, including the regulation of energy balance, immune system, and glucose metabolism (Deleu et al., 2021). Gut microbe that was favorable for the glycemic traits or T2D was associated with higher consumption of vegetables and fruits (Jiang et al., 2020; Wang et al., 2022a). Moreover, gut microbiota has been shown to mediate the effect of vegetables on the white blood cell profiles (Menni et al., 2021).

The beneficial role of vegetables and fruits may be owing to the contribution of fiber and flavonoids in diet-microbe cross-talk. Fiber is a non-digestible carbohydrate found in plants and provides the natural sources for fecal SCFAs. Therefore, high-fiber diet was commonly investigated in the context of disease treatment, such as inflammatory bowel disease (Wedlake et al., 2014; Deleu et al., 2021), cancer (Lam et al., 2021), and Type 2 diabetes (T2D) (Ren et al., 2018). Some randomized controlled trials involving fiber supplementation successfully altered microbial activity (i.e., SCFA production) and provided symptomatic relief or disease remission (Ren et al., 2018; Lam et al., 2021), however, some other trials failed (Wedlake et al., 2014; Deleu et al., 2021). Anthocyanins, a particular class of flavonoids, are enriched in fruits and vegetables, and could be metabolized by the intestinal microbiota (Faria et al., 2014; Boronat et al., 2021). Moreover, anthocyanins and their metabolites could enhance the growth of beneficial bacteria (e.g., Bifidobaterium and Lactobacillus) and reduce a group of potentially harmful bacteria, such as Clostridium histolyticum (Sánchez-Patán et al., 2012; Faria et al., 2014). Similar results in the Bifidobaterium have also been described in human volunteers after 6-week consumption of a blueberry drink (Vendrame et al., 2011). In addition, a recent study examined the microbial associations of circulating equol, a metabolite of dietary soy isoflavones, and found higher Alistipes senegalensis and Coprococcus catus but lower abundances of Ruminococcus gnavus were associated with equols (Wu et al., 2022b).

Polyphenol-rich foods, such as coffee and tea, could also affect the gut microbiome and influence host health. For example, coffee and tea were positively associated with Bifidobacterium, Lactobacillus, and Oscillibacter (Singh et al., 2017), and were inversely associated with proinflammatory pathways (Bolte et al., 2021). Tea consumption was inversely associated with unfavorable gut microbial profiles for T2D (Gou et al., 2021). Red wine polyphenols have been shown to modulate the gut microbiota (e.g., could increase F. prausnitzii and Roseburia; decrease E. coli and Bifidobacterium) and improve the metabolic profiles in healthy and obese participants (Queipo-Ortuño et al., 2012; Moreno-Indias et al., 2016; Bolte et al., 2021).

Refined grains showed different gut microbial associations from above healthy plant-based foods, and were associated with higher abundance of opportunistic bacterial genera such as F0332 and Terrisporobacter (Miao et al., 2022). In a 6-week randomized controlled trial, substituting whole grains for refined grains decreased levels of proinflammatory Enterobacteriaceae and increased SCFA-producing bacteria Lachnospira (Vanegas et al., 2017). It might be due to fewer fiber contained in the refined grains as they usually have seed coat removed. Previous studies also demonstrated that barely whole grains were more fermentable that refined wheat, and thus had a more effective SCFA production pattern (Nordlund et al., 2012; Vetrani et al., 2016).

Nuts and seeds also had a strong association with composition of gut microbiome. Roseburia hominis was positively associated with nuts intake, and showed beneficial effects on glucose metabolism by increasing butyrate production (Bolte et al., 2021). Enterococcaceae spp and Mollicutes RF39 spp had inverse associations with nuts intake and were potentially correlated to a unfavorable glycemic profile (Wang et al., 2022a; Miao et al., 2022). n-6 polyunsaturated fatty acids (PUFAs) is mainly enriched in nuts, a prospective cohort study shown that n-6 PUFAs was associated with lower microbial diversity and a higher risk of T2D, accordingly (Miao et al., 2020). It indicated that the beneficial association between nuts intake and glucose metabolism might be mediated by gut microbiota.

Consumption of total dairy and fermented dairy (e.g., yogurt) showed strong associations with high microbial alpha-diversity, lactic bacteria (Leuconostoc mesenteroides and Lactococcus lactis), and fermentation to butanediol pathway (Zhernakova et al., 2016; Bolte et al., 2021; Shuai et al., 2021; Yu et al., 2021). The consumption of milk was reported to be positively associated with the abundance of Streptococcus, Bifidobacterium, and Clostridium (Shuai et al., 2021). Of note, the dairy-Bifidobacterium association was consistently reported in several studies (Y. Zhernakova et al., 2016; Liu et al., 2019; Shuai et al., 2021). Dairy-favorable gut microbial features were inversely associated with blood triglycerides, while positively associated with high-density lipoprotein cholesterol (Shuai et al., 2021).

Several microbial associations have been identified for fish, including positive associations with R. hominis, Roseburia faecis, and F. prausnitzii (Bolte et al., 2021; Yu et al., 2021). Available evidence also highlights the potential importance of the dietary n-3 PUFAs, enriched in fish, to modulate the gut microbial composition. For example, after n-3 PUFA supplementation, a decrease in Faecalibacterium, and an increase in the Bacteroidetes and the production of SCFA have been observed (Costantini et al., 2017). Another cohort of 876 middle-aged and elderly women showed that n-3 PUFAs were correlated with microbial features belonging to Lachnospiraceae family, Ruminococcaceae family, and Bacteroidetes phylum (Menni et al., 2017). Nevertheless, a randomized cross-over trial including 22 middle-aged, healthy volunteers found that 8-week n-3 PUFA supplementation did not significantly change the gut microbial alpha-diversity, overall structure, or phyla abundance (Watson et al., 2017). On the other hand, n-3 PUFAs may interact with host genetics to affect gut microbiome and further link to diseases. A recent study found that higher n-3 PUFAs were prospectively associated with gut microbial features (diversity and genera) only among rs1527483-GG carriers, n-3 PUFAs-related gut microbial features were associated with blood lipids (Miao et al., 2022).

Red meat showed opposite taxonomic associations with above healthy plant-based foods. For example, Coprococcus, Bacteroides, Faecalibacterium, and Ruminococcus were inversely associated with processed red meat, and positively associated with fruit, whole grains, and fiber intake (Breuninger et al., 2021). Diet high in red meat may lead to the production of harmful bacterial metabolites such as TMAO leading to a disruption of the gut microbiota and increased risk of various diseases such as cardiovascular diseases and colon cancer (Koeth et al., 2013; Zaramela et al., 2019; Li et al., 2022).

Of note, other processed foods such as soft drinks and fast food had shown similar microbial associations with processed meat and were consistently linked to a higher abundance of Clostridium bolteae, Ruminococcus obeum, R. gnavus, and Blautia hydrogenotrophica (Breuninger et al., 2021). It indicated an impact of processed/ultra-processed foods on the human gut microbiome. Recent observational studies found that ultra-processed food intake was positively associated with Acidaminococcus, Butyrivibrio, Gemmiger, Shigella, Anaerofilum, Parabacteroides, Bifidobacterium in women, and with Granulicatella and Blautia in men (Cuevas-Sierra et al., 2021). Food additives, such as emulsifiers, which are commonly used in the processed foods, had a potential negative impact on the gut microbiota using mice and in vivo models (Chassaing et al., 2015; Naimi et al., 2021). Another kind of commonly used food additive artificial sweeteners may impact gut microbiota and human health as well. A randomized controlled trial performed among 120 healthy participants showed that oral supplementation with the sweeteners sucralose, saccharin, stevia, and aspartame induced perturbations of glucose tolerance, which might be mediated by compositional and functional changes in the gut microbiota (Suez et al., 2022). Currently, this field is severely under-researched in human epidemiology studies and further researches are needed to address the underlying mechanism of how food processing could influence gut microbiota and human health.

Collectively, these findings demonstrate that specific food types are closely correlated with gut microbial profiles and human health. Shared and distinct microbial associations have been reported for different food types.

Dietary patterns

Plant-based dietary pattern is growing in popularity due to its beneficial effects for human health, including reduced risk of T2D (Qian et al., 2019), cancer (Molina-Montes et al., 2020), and cardiovascular diseases (Kim et al., 2019). Early studies examined the impact of plant-based dietary pattern on the gut microbiome by characterizing the gut microbial composition of vegans or vegetarians compared with omnivores (De Filippis et al., 2016; Wu et al., 2016; Losasso et al., 2018). The vegetarian diets including vegan diets were reported to be associated with higher richness of gut microbiome and were associated with increased abundance of Bacteroidetes and decreased abundance of Firmicutes (Losasso et al., 2018). Vegetarian and vegan diets were also associated with higher abundance of Lachnospira and Prevotella in the gut, whereas omnivorous diets were associated with much lower levels (De Filippis et al., 2016). However, another US study found negligible differences in gut microbial community composition between vegans and omnivores in an urban environment (Wu et al., 2016).

Recent large-scale studies based on free-living individuals found that plant-based dietary pattern, as assessed using multiple 24-hour food recalls or food frequency questionnaires, were consistently associated with gut microbial alpha-diversity and overall composition (Asnicar et al., 2021; Bolte et al., 2021; Miao et al., 2022). These findings motivated the detailed exploration of plant-based diet-related microbial taxa and functional pathways. For example, habitual plant-based dietary pattern was associated with several commensals capable of SCFA production, including genus (Blautia, Ruminococcaceae UCG-009, and Polynucleobacter) and species (Agathobaculum butyriciproducens, F. prausnitzii, and Anaerostipes hadrus) (Asnicar et al., 2021; Miao et al., 2022). Consistently, Bolte et al. found the associations between plant-based foods and several carbohydrate fermentation pathways (Bolte et al., 2021).

Mediterranean diet (MedDiet) is characterized by high intakes of vegetables, fruits, nuts, and fish, and was associated with gut microbiome (De Filippis et al., 2016; Mitsou et al., 2017; Garcia-Mantrana et al., 2018; Merra et al., 2021; Wang et al., 2021a; Rinott et al., 2022). Using a longitudinal microbiome data, Wang et al. identified several gut microbial functional and taxonomic signatures for the MedDiet adherence (Wang et al., 2021a). At the taxonomic level, they found that F. prausnitzii, Eubacterium eligens, and Bacteroides cellulosilyticus were positively associated with MedDiet adherence. At the functional level, MedDiet adherence was associated with plant polysaccharide degradation, SCFA production, and pectin metabolism (Wang et al., 2021a). Moreover, in a 12-month randomized controlled trial including 612 participants, MedDiet could induce the enrichments of genus Prevotella and enzymatic functions involved in branched-chain amino acid degradation, and reduce the abundance of genus Bifidobacterium and enzymatic functions responsible for branched-chain amino acid biosynthesis (Ghosh et al., 2020). Those microbial features were also thought to mediate the effects of MedDiet on cardiometabolic health (Shankar Ghosh et al., 2020; Rinott et al., 2022).

A low-carb high-fat ketogenic diet is a diet that restricts carbohydrates intakes, primarily derived from sugary foods and refined grains, which could alter human gut microbiota. A short-term intervention with an isocaloric low-carb diet with increased protein content shifted the gut microbiota in obese participants with nonalcoholic fatty liver disease, including an increase in folate-producing Streptococcus and decreased fecal concentrations of SCFAs (Mardinoglu et al., 2018). Low-carb diet-associated gut microbiota could inhibit bifidobacterial growth, reduce the levels of intestinal inflammation and thus benefit metabolic health (Ang et al., 2020). Similar as the low-carb diet, low-fat diet was also demonstrated to be highly associated with gut microbiome. For example, a 6-month randomized controlled-feeding trial including 217 healthy young adults found that low-fat diet could increase gut microbial alpha-diversity, Blautia and Faecalibacterium, and reduce the blood proinflammatory markers (Wan et al., 2019).

Recently, dietary diversity has been linked to gut microbial profiles in the human cohort studies (Huang et al., 2022; Xiao et al., 2022). A diverse diet was consistently associated with a high microbial alpha-diversity, and a high abundance of Bacteroides ovatus, Turicibacter, Alistipes, and Barnesiella (Huang et al., 2022; Xiao et al., 2022). Nevertheless, data about the dietary diversity and gut microbiome is still sparse and more research in this field is warranted to further demonstrate the interplay between diversity of diet and gut microbiome profiles for host health.

Diet, nutrition, and microbiome across body sites beyond gut

Diet and nutrients could also affect the microbiota from other body sites, such as oral microbiota, vaginal microbiota, and human milk microbiota (Table 2). Oral microbiota also attracts attention given their close connection with diet and health (Lamont et al., 2018). Oral microbial alpha-diversity was positively associated with tea intake and inversely associated with total carbohydrates, glycemic load (GL), starch, lactose, and sucrose intake in US elderly populations (Peters et al., 2018; Millen et al., 2022). One study among Thailand children showed that frequently consumed snacks were associated with higher alpha-diversity of oral gut microbiota, which could predict early childhood caries (Wu et al., 2022a). Oral microbial beta-diversity was associated with tea intake, sweet treat consumption, total carbohydrates, fiber, GL, sucrose, and galactose (Peters et al., 2018; Lommi et al., 2022; Millen et al., 2022). Diet and nutrients were also associated with specific oral bacterial patterns (Esberg et al., 2021; Shaalan et al., 2022). For example, one study found that sucrose intake was associated with one oral bacterial pattern defined by the highest predicted sugar-related metabolic pathways and lowest species diversity, and caries status in Swedish adults (Esberg et al., 2021). Another study showed that consumptions of sugary snacks combined with reduced consumption of fish/shellfish and nuts were associated with one subtype of oral microbiome that was more represented in participants with T2D (Shaalan et al., 2022). For specific oral microbes, Peters et al. found that higher tea intake was associated with higher abundance of Fusobacteriales, Clostridiales, and Shuttleworthia satelles, and lower abundance of Bifidobacteriaceae, Bergeyella, Lactobacillales, and Kingella oralis. They also reported that higher coffee intake was associated with higher abundance of Granulicatella and Synergistetes, although it was not associated with alpha- or beta-diversity among the US elderly population (Peters et al., 2018). Higher sweet treat consumption was associated with higher abundance of Streptococcus, Prevotella, Veillonella, and Selenomonas, and associated with activated nitrate reduction IV and gondoate biosynthesis pathways among Finland children (Lommi et al., 2022). Thus, current evidence supports the associations of the intakes of tea, coffee, sugary foods, and carbohydrates with oral microbiota.

Human breast milk benefits infants’ immune system maturation and gastrointestinal development by providing nutrients, probiotics, and other bioactive molecules. Liu et al. showed that maternal diets were associated with the breast milk microbial composition in a Chinese population (Liu et al., 2022). For example, tuber was positively associated with the abundance of Neisseria and Cutibacterium in the breast milk; carbohydrate intake was inversely associated with the abundance of Aquabacterium; and vitamin B12 was positively associated with Coprococcus (Liu et al., 2022). Maternal consumption of fiber and fat, and mother’s infant feeding practice (frequency of direct breastfeeding) were associated with human milk microbiota (LeMay-Nedjelski et al., 2021). These studies suggest that the breast milk microbiota are influenced by maternal intake of tuber, fiber, and fat, as well as mother’s infant feeding practice.

The balance of vaginal microbiota was crucial for maintaining maternal-fetal health (France et al., 2022). Through investigating the effects of pre-pregnancy diet on vaginal microbiota composition, Dall’Asta et al. found that higher pre-pregnancy intakes of animal-sourced protein were inversely (harmfully) associated with a lactobacilli-dominated vaginal microbiota, while intakes of total carbohydrates and sugars were beneficial for healthy vaginal microbiota (Dall’Asta et al., 2021). Rosen et al. explored the associations between prenatal diet and vaginal microbiota composition and showed that the assumption of low-fat dairy was associated with a beneficial vagitype with predominance of Lactobacillus species (Rosen et al., 2022). These studies demonstrate the importance of pre-pregnancy (total carbohydrates and sugars) and prenatal diet (low-fat dairy) for healthy vaginal microbiota.

Microbial metabolites provide functional connection between diet, microbiome, and human health

Microbial metabolites act as important intermediates in the crosstalk between gut microbiota and host health (Fan and Pedersen, 2021; Krautkramer et al., 2021; Wang et al., 2022c). Microbial metabolites play essential roles in host metabolic homeostasis, immune functions, and neuromodulation (Fan and Pedersen, 2021; Krautkramer et al., 2021). Through producing a diverse array of metabolites, gut microbiota could even exert its functions in distant organs of human body, such as liver, lung, and brain (Van de Wouw et al., 2017; Tripathi et al., 2018; Dang and Marsland, 2019; Fan and Pedersen, 2021). The production of microbial metabolites depends, at least partially, on the availability of dietary substrate, in which bacteria ferment dietary macronutrients and micronutrients into functional metabolites. In this section, we summarize current epidemiological studies linking the associations between diet, microbial metabolites, and human health (Table 3).

TMAO is one of the most studied microbial metabolites, which is produced by the gut microbiota from foods rich in carnitine, phosphatidylcholine, and choline, such as red meat and eggs (Brown and Hazen, 2018; Dehghan et al., 2020). Leveraging longitudinal cohort from healthy US men, Li et al. showed that habitual intakes of red meat, egg, dairy, and fish were associated with increased plasma TMAO levels (Li et al., 2021). They further found that the positive association of red meat and choline with TMAO concentrations were only among participants with abundant TMAO-predicting species, suggesting the essential role of gut microbiota in TMAO production (Li et al., 2021). Consistently, a study based on the German population also showed that eggs and choline were positively associated with plasma TMAO levels (Rath et al., 2021). Furthermore, the detrimental relationships between TMAO levels and cardiovascular diseases have been manifested in many studies (Senthong et al., 2016; Li et al., 2017; Yu et al., 2019; Lee et al., 2021; Wei et al., 2022). The associations of diet and nutrient with cardiovascular diseases may depend on the TMAO levels. For example, a previous study showed that TMAO-related metabolites (TMAO, γ-butyrobetaine, and crotonobetaine) together mediated the associations of unprocessed red meat (mediated proportion: 10.6%), total meat (mediated proportion: 7.8%), and total animal-source foods (mediated proportion: 9.2%) with risk of atherosclerotic cardiovascular disease (Wang et al., 2022b). Another study demonstrated that the associations of high levels of plasma choline and betaine with major adverse cardiac events (MACE) were significant only among those with concomitant elevated TMAO levels, while neither choline nor betaine was associated with risk of MACE when adjusting for TMAO levels (Wang et al., 2014).

Tryptophan, an essential amino acid, is beneficial for human health, which can be converted to a variety of indole derivatives (e.g., indoleacetate, indolelactate, and indolepropionate) by gut microbiota (Roager and Licht, 2018). Qi et al. showed that indolelactate was positively while indolepropionate was inversely associated with risk of T2D (Qi et al., 2022). They also found that higher fiber intake was associated with higher levels of indolepropionate, and lower levels of indolelactate and indoxyl sulfate. As a support, one recent intervention study found that a prebiotic fiber supplement could significantly increase plasma indolepropionate concentrations compared to the placebo among healthy young participants consuming a diet low in fiber (Kang et al., 2022). Higher intakes of vegetables, fruits, whole grains, nuts, and legumes, and lower intakes of refined grains and red meat were associated with higher serum indolepropionate levels (Qi et al., 2022). Fruit and vegetable intakes were also inversely associated with urinary 3-indoxylsulfate concentrations (Szabo de Edelenyi et al., 2021).

Imidazole propionate (ImP) is a microbial metabolite from histidine. Serum ImP levels were higher in participants with prediabetes or T2D, and this metabolite was also associated with gut microbial enterotype and gene richness (Molinaro et al., 2020). In addition, ImP levels were positively associated with saturated fat intake (driven by high cheese intake), and negatively associated with fiber and unsaturated fat intake (driven by reduced intake of vegetables and nuts) (Molinaro et al., 2020). One intervention study conducted by Nishimoto et al. demonstrated that the intake of resistant maltodextrin (one of the dietary fibers) could reduce fecal ImP levels among Japanese participants (Nishimoto et al., 2022). For the dietary pattern, serum ImP levels were inversely associated with the alternate Healthy Eating Index, dietary diversity score, and MedDiet score (Molinaro et al., 2020). Overall, dietary fiber, unsaturated fat intake, and healthy dietary patterns may be inversely associated with T2D and glucose metabolism disorder through reducing the levels of ImP.

SCFAs, including acetic acid, propionic acid, and butyric acid are produced by microbial fermentation of dietary fiber. The evidence for the associations of SCFAs with diet and health primarily comes from rodent studies (Krautkramer et al., 2021). In human studies, Cuesta-Zuluaga et al. showed that higher fecal SCFA concentrations were associated with gut permeability, markers of metabolic dysregulation, obesity, and hypertension (de la Cuesta-Zuluaga et al., 2018). Utilizing the bidirectional Mendelian randomization analysis, Sanna et al. demonstrated that high levels of fecal butyrate was causally associated with improved insulin sensitivity, while higher levels of fecal propionate were causally associated with higher risk of T2D (Sanna et al., 2019). In addition, higher serum propionic acid levels were associated with increased risk of cognitive decline and were positively correlated with intakes of meat and cheese (Neuffer et al., 2022).

Bile acids (BAs), which are synthesized from cholesterol in the liver, act as important intermediates in gut microbiota-host crosstalk. A cross-sectional study showed that serum primary and glycine-conjugated BAs in vegans were higher but all fecal BAs were lower than omnivores (Trefflich et al., 2019). Through deriving dietary patterns, this study also showed that fat intake was positively, while fiber intake was inversely associated with BAs levels (Trefflich et al., 2019). In addition, Jiang et al. found that fruit-related gut microbiota index was inversely associated with fecal cholic acid and 3-dehydrocholic acid, which were positively associated with T2D risk (Jiang et al., 2020). Another study discovered that habitual tea consumption was inversely associated with chronic insomnia-disrupted BA norcholic acid (Jiang et al., 2022), suggesting the potential role of habitual tea consumption in treating chronic insomnia through targeting BAs. Meanwhile, Wang et al. systematically explored the associations between diet, microbial genetics, and plasma BA composition, providing a valuable resource for future studies (Wang et al., 2021b).

Except for the TMAO, there are other microbial metabolites that belong to gut-derived uremic toxins (GDUT), including p-cresyl sulfate, hippuric acid, p-cresyl glucuronide, pheny acetyl glutamine, and phenyl sulfate. Plasma levels of GDUT were all significantly higher even with moderate reduction of renal function (Pignanelli et al., 2019). Intake of meat/amino acids contributed to plasma abundances of all GDUT. Plasma levels of hippuric acid and p-cresyl gluronide were significantly explained by intakes of TMA, largely from egg yolk (Pignanelli et al., 2019). Thus, participants with impaired renal function should limit the intake of red meat, animal protein, and egg yolk. In addition, intestinal microbial metabolites of dietary lignans such as enterolactone and enterodiol were associated with modestly slower weight gain (Hu et al., 2015).

Apart from above studies, several recent studies have systematically explored the associations of diet and gut microbiome with more than 1000 metabolites (Bar et al., 2020; Chen et al., 2022), providing important resources for potential diet-related microbial metabolite investigations in future.

Role of gut microbiome in the precision nutrition research

Precision nutrition is a broad concept to answer “What to Eat to Stay Healthy,” covering multidisciplinary integration, among which, gut microbiome is becoming an essential component (Rodgers and Collins, 2020). In the above sections, we have summarized how dietary pattern, food groups, or nutrients affect the gut microbiota diversity, taxa, and functions across different cohorts or clinical studies. Here we review another important area of gut microbiome-based precision nutrition for the identification of key microbiota features that predict the host phenotypic response to diet, which can then inform the design of microbiome-guided personalized nutrition guidelines for diverse individuals (Fig. 2 and Table 4).

Similar as the application of nutrigenetics in the precision nutrition, a number of studies have consistently found that stratifying individuals according to gut microbial enterotypes (dominance of either Prevotella or Bacteroides) is applicable, which is the initial application of gut microbiome-based precision nutrition research (Hjorth et al., 2018; Hjorth et al., 2019). For example, a 26-week randomized controlled trial showed that participants with Prevotella-dominated microbial enterotype lost more body weight on a high-fiber diet intervention than a western diet intervention, whereas no difference in body weight change was observed for the participants with Bacteroides-dominated microbial enterotype (Hjorth et al., 2018). Consistently, another 24-week dietary intervention study found that participants with high Prevotella-to-Bacteroides ratio lost more body weight and body fat compared to participants with low Prevotella-to-Bacteroides ratio (Hjorth et al., 2019). Participants with a high Prevotella-to-Bacteroides ratio might result in improvement of enzymatic capacity for fiber digestion and glucose metabolism (Hjorth et al., 2019). Microbial gene richness is another important microbial index for the subgroup-based precision nutrition. A 12-week weight-maintenance diet intervention study included 38 obese and 11 overweight participants, and found that the effect of weight-maintenance dietary intervention on the clinical phenotypes was less pronounced among participants with lower gut microbial gene richness (Cotillard et al., 2013).

Gut microbiome is a major factor determining the variation of individual’s metabolic response to diet, which provides a rationale to combine the gut microbial features and other host phenotypes to predict the host response for similar or different dietary challenges. A landmark study within this field was the Israeli personalized nutrition study (n = 800) (Zeevi et al., 2015). They found that the combination of gut microbiome, dietary factors, anthropometrics, and clinical parameters could accurately predict personalized postprandial glycemic response to real-life meals. Furthermore, 1-week personalized dietary interventions based on the prediction showed improvement in glucose metabolism. As a validation of this study, Ben-Yacov et al. randomly assigned 225 adults with prediabetes to follow a MedDiet or a machine learning-predicted personalized postprandial-targeting (PPT) diet for a 6-month intervention and additional 6-month follow-up (Ben-Yacov et al., 2021). They found that a PPT diet improved glycemic control more effectively than a MedDiet (Ben-Yacov et al., 2021). With the same study design and predictive model as the Israeli study, Mendes-Soares et al. enrolled 327 participants without diabetes from the USA. They found that the postprandial response to the same foods varied across participants, and the modeling framework developed by the Israeli study could be applicable to a Midwestern population (Mendes-Soares et al., 2019). Inspired by the Israeli personalized nutrition study, a large-scale twin study recruited more than 1000 healthy adults from the UK to USA and undertook a 2-week interventional trial. They observed that even genetically similar twins had differently postprandial responses of blood triglycerides and glucose to identical meals. They highlighted that environmental factors, mainly gut microbiome could predict the triglycerides and glycemic responses to diet (Berry et al., 2020).

For a specific disease population, like inflammatory bowel syndrome (IBS) patients, a 4 weeks intervention study included 67 participants who were randomized to traditional IBS (n = 34) or low fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) (n = 33) diets (Bennet et al., 2018). They found that low FODMAPs, but not traditional IBS diet responders could be discriminated from non-responders based on gut microbiome before the intervention (Bennet et al., 2018).

Indeed, even for the specific food groups or nutrients, their different subtypes may have personalized effects on the human metabolism, which could be predicted by gut microbiome. Korem et al. performed a crossover clinical trial for the consumption of industrially made white bread or artisanal sourdough-leavened whole-grain bread (Korem et al., 2017), and found that glycemic response to different bread types varies greatly across individuals, and the type of bread that induces the lower glycemic response in each person can be predicted based on the gut microbial features (Korem et al., 2017). Deehan et al. showed that small differences in dietary fiber structure could have distinct effects on the gut microbiome composition, leading to directed shifts in the output of either propionate or butyrate (Deehan et al., 2020). Suez et al. found that different type of non-nutritive sweeteners personalized altered stool and oral microbiome. Only saccharin and sucralose were causally linked to impaired glycemic responses (Suez et al., 2022).

Conclusions and future perspectives

In this review, we have discussed the latest advances in understanding the role of the microbiome in nutrition and human health. Microbiome-based nutritional (i.e., nutri-microbiome) epidemiological studies have uncovered many microbes and microbial metabolomic targets for dietary intervention and disease prevention. Furthermore, the integration of nutrition with microbiome provides new approach for understanding the mechanism behind the diet-disease associations. In particular, inter-individual differences in postprandial metabolic responses to diet challenge the logic of “one-size-fits-all” dietary recommendations. Gut microbiome have been demonstrated as one of the effective predictors of host response to a particular diet. Gut microbiome-based precision nutrition provides hope for further advancements in control and treatment of disease at individual level.

The advances in nutri-microbiome epidemiological studies are promising, yet, there are several limitations in this field, including study design, technologies, analysis methods, and results interpretation. First, the nutrition and microbiome associations are generally studied in cross-sectional or short-term longitudinal settings, while the human gut hosts a dynamic microbial ecosystem which is continuously perturbed by daily dietary intake (Johnson et al., 2019; Olsson et al., 2022). Therefore, well-designed longitudinal investigations with repeat measures, ideally randomized controlled trials, are needed to assess diet-gut microbiota interaction on the human health. Second, geographic location, customs, and culture, seasonal variations in food availability could all impact the dietary choices and the gut microbiome heterogeneity (Ecklu-Mensah et al., 2022), and therefore caution should be taken in extrapolating findings from different geographic location or ethnic groups. Third, mass spectrometry metabolomic profiling could detect thousands of unique signal features. However, it is currently difficult to accurately identify metabolites for many of these signal features, which affects the discovery of new metabolomic biomarkers. Finally, machine learning has been widely and effectively applied in the precision nutrition studies, while its prediction algorithms are usually difficult to interpret.

Despite several limitations and obstacles, recent advances in the field bode well for the future. The repeated-crossover design of an n-of-1 trial is an advantageous approach to compare the effect of two or more interventions for an individual (Gabler et al., 2011; Potter et al., 2021). Nutritional n-of-1 clinical trials, in the context of the human gut microbiota provide a unique opportunity to characterize the host response and gut microbial variability of nutritional interventions at the individual level (Zheng and Ordovás, 2021). Mobile apps and wearable devices facilitate the real-time assessment of dietary intake, nutritional biomarker variations, and changes of health-related vital signs (Sempionatto et al., 2021). Current advances in wearable devices have proved prerequisite for precision nutrition (Sempionatto et al., 2021; Merino et al., 2022). Furthermore, interpretable machine learning has received more and more attentions in recent years (Murdoch et al., 2019). By integrating cutting-edge wearable and mobile sensing technologies with interpretable machine learning, precision nutrition is expected to provide effective personalized nutrition guidance and interventions. The applications of metabolomics in the field of nutrition and microbiome could greatly benefit from the improvement of the stability and repeatability of metabolomic profiling, the standardization of analytical methods and strategies, the advances in metabolic peak identification, the comprehensive utilization of different biological samples for metabolomic profiling. Moreover, research on the interactions of the gut multi-kingdom ecosystem (including bacteria, virome, and mycobiome community, etc.) with diet and nutrition for human health is an emerging field of great interest.

To conclude, systematic integration of microbiome with the large-scale nutritional epidemiological cohort studies fosters the development of nutri-microbiome epidemiology, a rising field to disentangle the relationship among diet, nutrition, microbiome, and human health. These new progresses stimulate the further development of microbiome-based precision nutrition research.

Abbreviations

 
• BAs

  bile acids

 
• FODMAPs

  fermentable oligosaccharides, disaccharides, monosaccharides, and polyols

 
• GDUT

  gut-derived uremic toxins

 
• GL

  glycemic load

 
• IBS

  inflammatory bowel syndrome

 
• ImP

  imidazole propionate

 
• MACE

  major adverse cardiac events

 
• MedDiet

  Mediterranean diet

 
• PPT

  personalized postprandial-targeting

 
• PUFAs

  polyunsaturated fatty acids

 
• SCFA

  short-chain fatty acids

 
• TMAO

  trimethylamine N-oxide;

 
• T2D

  , Type 2 diabetes

Funding

This study was funded by the National Key R&D Program of China (No. 2022YFA1303900), National Natural Science Foundation of China (Nos. 82204161, 82073529), the Research Program (No. 202208012) of Westlake Laboratory of Life Sciences and Biomedicine, and China Postdoctoral Science Foundation (2022M722833, 2022M710131).

Conflict of interest

Wanglong Gou, Zelei Miao, Kui Deng, and Ju-Sheng Zheng declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by the any of the authors.

Consent for publication

Written consent is available for review by the Editor-in-Chief of this journal.

Data availability

No dataset was generated or analyzed during this study.

Authors’ contributions

All authors researched the data for this article, made substantial contributions to discussion of the content, and wrote, reviewed, and edited the manuscript before submission.

References

Author notes