-
Views
-
Cite
Cite
Hiromichi Morikawa, Masanori Asada, Yasuyuki Yamada, Effects of Physical Parameters upon Protoplast Electrofusion, Plant and Cell Physiology, Volume 29, Issue 4, June 1988, Pages 659–664, https://doi.org/10.1093/oxfordjournals.pcp.a077543
- Share Icon Share
Abstract
The effects of three physical parameters upon protoplast electrofusion were studied using protoplasts from cultured cells of Coptis japonica and Euphorbia millii. The osmotic potential of the medium did not appreciably affect the AC-field-induced protoplast-pair formation, but significantly influenced the fusion process of the paired protoplasts in response to DC pulses. The optimum osmotic potential was 0.55 to 0.60 Osm/kg H2O in our system. The density of the medium markedly influenced both pair formation and fusion process. The optimum density was 1.13 to 1.14 g/cm3, and at this density the yield of the fused protoplasts increased to more than twice that of the control (1.10 g/cm3). Hydrophilic coating of the bottom surface of the chamber with Gellan gum or polyacrylamide gel was also effective for both pair formation and the fusion process, while coating with hydrophobic silicone was entirely inhibitory. Possible interpretations of the effects of these physical parameters upon protoplast electrofusion are presented.