FP046
GENERATION OF KIDNEY ORGANOIDS FROM BONE MARROW-DERIVED MESENCHYMAL STEM CELLS

INTRODUCTION AND AIMS: Progenitors can be induced to both collecting duct and nephrons via the directed differentiation of stem cells. The human kidney originates from intermediate mesoderm. Cells cephalad migrate from the primitive streak (presomitic mesoderm) to form the intermediated mesoderm. The intermediate mesoderm increases the number of key renal progenitor cells, as well as the ureteral epithelium and the metanephric mesenchyme, which respectively form the collecting ducts and nephrons. Here we have used bone marrow-derived mesenchymal stem cells (BM- MSCs) to differentiate into the kidney.

METHODS: The kidney organoids were generated from MSCs with CHIR99021-FGF9 cytokines, and the kidney specific markers in the kidney organoids were detected by immunofluorescence staining. The electron microscope was used to observe the structure of the organoids.

RESULTS: The essential cytokines is required for the MSCs to differentiate into the kidney organoids. The generated kidney organoids are comprised of anticipated kidney cell types including nephrons segmented into the glomerulus, proximal tubule, as well as the collecting duct, and blood vessels.

CONCLUSIONS: Such kidney organoids generated from MSCs represent powerful models of the human organ for future applications, including nephrotoxicity screening, disease modelling and as a source of cells for therapy