SP199
THE ITRAQ TECHNOLOGY IDENTIFIES BIOMARKERS FOR THE EARLY DIAGNOSIS OF CONTRAST&#8722INDUCED ACUTE KIDNEY INJURY Free

INTRODUCTION AND AIMS: This study was to detect differentially expressed urine proteins in contrast-induced acute kidney injury (CI-AKI) patients after percutaneous coronary intervention (PCI) by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology and Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) technology, and to find new biomarkers for early diagnosis of CI-AKI.

METHODS: We collected urine samples of patients before PCI and at 6hrs after PCI from July 1st 2015 and December 31st 2015. Blood collected for biochemical analyses and Scr concentrations.ITRAQ technique combined with LC-MS/MS were used to screen differentially expressed proteins, and to identify potential biomarkers by analysis of the biological process, cellular components, molecular functions, Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways and protein-protein interactions(PPI).

RESULTS: Eighty two patients admitted for elective PCI were included in the study. Among these patients, 6 (4 male cases and 2 female cases) developed CI-AKI. Comparing to patients before PCI, a total of 151 proteins were identified in the CI-AKI group (6hrs after PCI). 74 proteins were up-regulated and 77 proteins were down-regulated. We identified 20 biological process, 20 cellular components, 20 molecular functions and 10 significant KEGG pathways. Combined with the PPI results, mannan-binding lectin (MBL)-associated serine protease 2 (MASP2), angiotensinogen (AGT) and apolipoproteins A-I(apoA-I) might play a role in the pathogenesis of CI-AKI.

CONCLUSIONS: 1.The quantitative iTRAQ technology provided an accurate and effective assessment of identifying and profiling potential urine biomarkers for early diagnosis of CI-AKI in this study.2.Our research showed that MASP2, AGT, and apoA-I were significantly up-regulated at 6hr after PCI. And they were earlier than Scr for diagnosis of CI-AKI. They were potential urine biomarkers and played key roles in the pathogenesis of CI-AKI.