SP168
PRESENCE OF CAMPYLOBACTER RECTUS AND CNM-POSITIVE STREPTOCOCCUS MUTANS STRAINS IN ORAL CAVITY WAS ASSOCIATED WITH URINARY PROTEIN LEVELS IN IGAN PATIENTS

INTRODUCTION AND AIMS: Some bacterial species have been reported to be the candidate of the antigen or the pathogenesis of IgAN. Dental caries is one of the two major diseases in the dentistry field, and Streptococcus mutans is known to be a major pathogen. We demonstrated that cnm-positive S. mutans was associated with the urinary protein levels in IgAN patient (Scietific Rep, 2016). Periodontitis is another major disease in the field of dentistry. Recently, we performed the analysis of tonsil flora in IgAN patients, which revealed that the periodontitis-related pathogens Campylobactor rectus or Treponema species were demonstrated to be associated with IgAN. Moreover, we found the remission rates of proteinuria and hematuria were significantly related with the prevalence infection of C. rectus and Treponema species (Plos One,2014). In the present study, we focused on periodontitis-related pathogens C. rectus and T. denticola as well as S. mutans, a pathogen of dental caries to understand the association of IgAN and these bacterial species using the saliva specimens from the patients with clinical parameters related to IgAN.

METHODS: We evaluated the results of cnm-positive S. mutans in the saliva specimens and clinical data of each subject. The saliva specimens used to evaluate the presence of C. rectus and T. denticola DNA by PCR. We could get the information of the 117 IgAN patients who are outpatients of Seirei Hamamatsu General Hospital, Hamamatsu, Japan. The IgAN patients were diagnosed by the previous renal biopsies. The clinical data were collected at the time of informed consent. Age-matched healthy subjects (n=56) were included as the non-diseased control group.

RESULTS: The detection rate of C. rectus and cnm-positive S. mutans were shown to be significantly more prevalent in the IgAN group than in the control group (P<0.05). The positive rates of C. rectus were significantly more prevalent in the IgAN group than in controls. Although we could not show the significant differences of the urinary protein levels (g/gCr) between the C. rectus-positive and -negative groups (p=0.0618), it was demonstrated that % Urinary protein 1 + or higher in the C. rectus-positive group were significantly higher than that in the C. rectus-negative group in IgAN patients. In addition, both C. rectus-positive and cnm-positive S. mutans group were significantly associated with urinary protein levels than other groups in IgAN patients (Data were summarized in Table).

CONCLUSIONS: Our results suggest that C. rectus and/or cnm-positive S. mutans strains in the oral cavity were associated with urinary protein levels in the IgAN patients.