This is a correction to: Seong Jun Cho, Jin Zhang, Xinbin Chen, RNPC1 modulates the RNA-binding activity of, and cooperates with, HuR to regulate p21 mRNA stability, Nucleic Acids Research, Volume 38, Issue 7, 1 April 2010, Pages 2256–2267, https://doi.org/10.1093/nar/gkp1229.
In the original publication of this article, the GAPDH/IP:αHuR, GAPDH/IgG and p21/IgG blots in Fig. 6D were accidentally duplicated. The error occurred during figure assembly.
The authors have provided the original raw data (Supplementary file 1) and a new Fig. 6, shown below.
RNPC1 enhances the RNA-binding activity of HuR in vivo. (A) RKO cells were uninduced or induced to express HA-tagged RNPC1a for 16 h, and then used for immunoprecipitation with anti-HA to capture RNPC1–RNA complexes, anti-HuR to capture HuR–RNA complexes, or mouse IgG as a control. Total RNAs were purified from immunocomplexes and subjected to RT–PCR to measure the level of p21 transcript associated with RNPC1a or HuR. The level of GAPDH was also measured as a control. (B) The level of p21 transcript associated with RNPC1- or HuR-immunocomplexes was quantified by qRT–PCR. *P < 0.01 (Students’ t-test). (C) The level of RNPC1a was measured by RT–PCR in MCF7 cells uninduced (−) or induced (+) to knock down RNPC1a for 3 days. (D) MCF7 cells were uninduced or induced to knockdown RNPC1a for 3 days, and then used for immunoprecipitation with anti-HuR to capture HuR–RNA complexes or mouse IgG as a control. Total RNAs were purified from immunocomplexes and subjected to RT–PCR to measure the level of p21 transcript associated with HuR. The level of GAPDH was also measured as a control.
This correction does not affect the results, discussion and conclusions presented in the article. These details have been corrected only in this correction notice to preserve the published version of record.
Supplementary data
Supplementary data is available at NAR online.
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