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Journal Article
ACCEPTED MANUSCRIPT
Jess C Hercus and others
Molecular Human Reproduction, gaaf015, https://doi.org/10.1093/molehr/gaaf015
Published: 26 April 2025
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Published: 26 April 2025
Graphical Abstract
Journal Article
ACCEPTED MANUSCRIPT
Xiangyu Chang and others
Molecular Human Reproduction, gaaf014, https://doi.org/10.1093/molehr/gaaf014
Published: 25 April 2025
Journal Article
ACCEPTED MANUSCRIPT
Gee Soo Jung and others
Molecular Human Reproduction, gaaf013, https://doi.org/10.1093/molehr/gaaf013
Published: 15 April 2025
Journal Article
Molecular Human Reproduction, Volume 31, Issue 2, 2025, gaaf010, https://doi.org/10.1093/molehr/gaaf010
Published: 10 April 2025
Journal Article
ACCEPTED MANUSCRIPT EDITOR'S CHOICE
Andrew Cearlock and others
Molecular Human Reproduction, gaaf012, https://doi.org/10.1093/molehr/gaaf012
Published: 07 April 2025
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Published: 18 March 2025
Figure 1. Protein-targeting reverse genetic approaches . ( A ) Trim-Away: the E3 ligase and antibody binding protein TRIM21 is expressed in cells. In oocytes, eggs, and preimplantation embryos, this is achieved through mRNA injection or electroporation. An antibody that specifically interacts with the POI is
Journal Article
Nicole J Camlin
Molecular Human Reproduction, Volume 31, Issue 2, 2025, gaaf008, https://doi.org/10.1093/molehr/gaaf008
Published: 18 March 2025
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Published: 18 March 2025
Figure 2. Protein-targeting approaches allow temporal control of protein function . ( A ) Stages of oocyte maturation and early preimplantation embryo development. Oocytes resume meiosis exiting prophase I arrest before progressing through oocyte maturation to arrest again as eggs at metaphase II. These eggs
Journal Article
Yashuang Weng and others
Molecular Human Reproduction, Volume 31, Issue 2, 2025, gaaf007, https://doi.org/10.1093/molehr/gaaf007
Published: 14 March 2025
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Published: 14 March 2025
Figure 4. Effects of human platelet-rich plasma on the activation of PI3K/Akt pathways in cultured mouse ovaries . Ovaries from 3-day post-partum mice were cultured in either standard medium (control group) or medium supplemented with 2% hPRP (hPRP group), or 5 mM LY294002 (LY group), or with 2% hPRP and 5 mM
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Published: 14 March 2025
Figure 6. Effects of human platelet-rich plasma on mouse primordial follicle activation in vivo . Female mice at 3 days post-partum were injected i.p. with 2 ml/kg hPRP twice daily for two consecutive days. In the control group, female mice were injected with PBS solution. Ovaries were collected at 2 days (
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Published: 14 March 2025
Figure 3. Subsequent development of follicles activated by human platelet-rich plasma . ( A ) The timeline of the experiment is shown. Ovaries from 3-day post-partum mice were cultured either in medium for 8 days (control group) or in medium supplemented with hPRP for 4 days, followed by an additional 4 days
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Published: 14 March 2025
Figure 5. Effects of human platelet-rich plasma on the transcriptome in cultured mouse ovaries . Ovaries from 3-day post-partum mice were cultured in either standard medium (control) or medium supplemented with 2% hPRP (hPRP group) for 6 h. ( A ) Volcano plot illustrating differentially expressed genes betwee
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Published: 14 March 2025
Figure 1. Human platelet-rich plasma promotes primordial follicle activation in cultured mouse ovaries . Three day post-partum newborn mouse ovaries were cultured in medium supplemented with different concentrations of hPRP (0%, 1%, 2%, and 5%). ( A ) After 4 days of culture, ovaries were subjected to morphol
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Published: 14 March 2025
Figure 2. Human platelet-rich plasma promotes granulosa cell proliferation in cultured mouse ovaries . Ovaries from 3-day post-partum mice were cultured in control medium or medium supplemented with 2% hPRP for 1 day. ( A ) Immunofluorescence staining was performed in the control and hPRP groups after 1 day o
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Published: 14 March 2025
Figure 7. Effects of human platelet-rich plasma on human primordial follicle activation in vitro . Human ovarian fragments were directly collected for histological analysis and protein detection (uncultured). Human ovarian fragments were cultured in either standard medium (control) or medium supplemented wi
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Published: 12 March 2025
Figure 7. Comparison of the developmental zygotic events occurring between sperm injection and first mitosis. ( A ) Illustration of the zygotic events observed between sperm injection and first mitosis: second polar extrusion (second PB), appearance of the male pronucleus (male PN), appearance of the female
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Published: 12 March 2025
Figure 5. Impaired development of embryos obtained by ICSI with sperm from MMAF lineages and WT oocytes. ( A ) Developmental curves showing the successive arrests of embryo development between sperm injection and blastocyst formation. The number of embryos produced with WT sperm (228), with sperm from Armc2
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Published: 12 March 2025
Figure 1. Morphological nucleus defects and their occurrence in four MMAF lineages. Head morphologies were analyzed with the nuclear morphology analysis software and 24 different sperm head shapes (shape #1 to shape #24) were identified by the software from WT and deficient males. The percentages of each sha