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Sidney Glina, William J. Sommers, Andrew R. McCullough, Classic Citations: Lue TF, Zeineh SJ, Scmidt RA and Tanagho EA—Neuroanatomy of Penile Erection: Its Relevance to Iatrogenic Impotence, The Journal of Sexual Medicine, Volume 8, Issue 2, February 2011, Pages 337–340, https://doi.org/10.1111/j.1743-6109.2010.02186.x
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Lue et al. in this landmark paper [1] investigated the anatomy of cavernous nerves by humans cadaveric dissection and serial histologic sectioning in dogs. Their objective was to establish the anatomy of the “nervi erigentes” from the spinal center to the penis and their relationship to the rectum, prostate, and urethra.
Their group had a great experience in isolating the cavernous nerves for acute and chronic electrical stimulation of the penis in dogs and monkeys, and in previous studies they found that the protein tracer horseradish peroxidase was a very useful agent to localize bladder and urethral nuclei through retrograde axonal transport [2].
In this study, they injected 0.3 cc of 30% horseradish into the corpora cavernosa of six mongrel dogs (group 1). In another three dogs (group 2), the pelvic organs were surgically exposed. The cavernous nerves were identified through their location and electrical stimulation and transected. The proximal stump was bathed in horseradish and dimethylsulfoxide for 6 hours. All dogs were sacrificed 72 hours later, and the spinal cord from T6 to the cauda equina was fixed, the tissue was cut in serial 32 µm sections and processed for horseradish peroxidase reaction.
