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I. Sokolakis, F. Dimitriadis, D. Psalla, G. Karakiulakis, G. Hatzichristodoulou, D. Kalyvianakis, D. Hatzichristou, PS-04-005 Low Intensity Shockwave Therapy (LiST) may Promote Angiogenesis and alter a1/a2 Adrenergic Receptors Ratio in the Erectile Tissue of Naturally Aged Rats, The Journal of Sexual Medicine, Volume 16, Issue Supplement_2, May 2019, Page S12, https://doi.org/10.1016/j.jsxm.2019.03.065
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Objective
To investigate the effects of LiST on erectile tissue of healthy, naturally aged rats.
Methods
Twelve naturally aged male Wistar albino rats (19-21 months) were randomized into two groups: a control group (OC, n=6) and a LiST treatment group (OSWT, n=6). Another control group of young rats (8 weeks) (n=6) was also used (YC). Each rat in OSWT group received 300 shockwaves with an energy flux density of 0.09mJ/mm2 at 2 Hz, at the middle of the penile shaft. Sessions were repeated three times/week for two weeks, followed by a two-week washout period. Penile tissues were harvested and analysed with real time reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemical analysis (IHC). Outcomes: The mRNA expressions of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), nerve growth factor (NGF) and neuronal NOS (nNOS), as well as α1 and α2-adrenergic receptors (α1AR, α2AR). Further IHC to evaluate the protein expression for the statistical significant mRNA expressions was performed.