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A. Sytenko, I. Gorpynchenko, PS-04-005 Intracavernous Injections of Bone Marrow Mesenchymal Stem Cells Restore Phosphatase Activity of Cavernous Tissue in Male Streptozotocin-Diabetic Rats, The Journal of Sexual Medicine, Volume 14, Issue Supplement_4a, April 2017, Page e117, https://doi.org/10.1016/j.jsxm.2017.03.102
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Objective
To assess the impact of intracavernous injections (ICI) of bone marrow mesenchymal stem cells (BMMSCs) on alkaline phosphatase (AP) and ATP-ase activity in cavernous tissue (CT) from male streptozotocin (SZC) diabetic rats.
Methods
Effects of II of BMMSCs were studied in 30 sexually matured male Wistar rats (160 - 180 g) with SZC-induced hyperglycemia (glucose level> 8 mmol /l ) lasting >3 months. Animals were randomly distributed between the two groups (1:1): Stem group (SG) (n=15) were to receive 4 ICI of 1 ml suspension of BMMSCs (1*10ˆ6), Placebo grope (PG) got placebo in the same mode. Specimens of CT were harvested before (n=5 from each group) and after (n=10) the course of ICI. Enzyme activity in CT was assessed by means of Gomori reaction for AP and Maizel reaction for ATP-ase.
Results
The average blood glucose level in SG before starting after completing the ICI was 35+-8 mmol / l and -37+-9 mmol / l, respectively. In PG: 36+-7 mmol / l and -39+-11 mmol / l. As well before ICI in each group a marked reduction of both AP and ATP-ase activity in the endothelial lining of the sinusoids and arterial vessels of CT was registered. After 1 months of treatment only in SG partial restoration of these enzymes activity was observed.