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Kazuhiro Aoyama, Ryusuke Kuwahara, Kana Yokoyama, Kaoru Mitsuoka, C6-P-02
Cryo-STEM Tomography for Cell structure Analysis , Microscopy, Volume 64, Issue suppl_1, November 2015, Page i141, https://doi.org/10.1093/jmicro/dfv333 - Share Icon Share
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Recently, STEM tomography has started to be applied for biological specimens. STEM tomography has several important advantages compared with conventional TEM method.
1) Clear imaging for thick specimens
2) Dynamic focusing
3) Easy to select high-contrast imaging mode
4) Linear contrasts
5) Wide depth of field by small convergence angle
In this study, the technique applied for Cryo-specimens. Of course, even in Cryo-Tomography, the advantages of STEM above mentioned are valid. Because STEM has advantage to resist specimen thickness, it is expected to be powerful method for observing whole cell structure in Cryo-microscopy without thin sectioning.
The insufficient contrast is one of the serious problems in Cryo-electron microscopy. Therefore, the image contrasts by TEM and STEM have to be compared carefully and quantitatively. The result was clear that the STEM image had very low back ground noise. This character can be explained theoretically, there are several seasons; (1) Short operation time for pixel (dual time vs. exposure time), (2) Large physical size of the detector, (3) Very small collection angle (same as very small objective aperture in TEM imaging).