1-20 of 11483
Journal Article
Live-cell omics with Raman spectroscopy
Microscopy, dfaf020, https://doi.org/10.1093/jmicro/dfaf020
Published: 24 April 2025
Journal Article
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High energy-resolution soft X-ray emission spectrometer using a back-thinned CMOS detector for chemical bonding state analysis. Get access
Microscopy, dfaf021, https://doi.org/10.1093/jmicro/dfaf021
Published: 16 April 2025
Journal Article
ACCEPTED MANUSCRIPT
Ultra-Low Accelerating Voltage Scanning Electron Microscopy with Multiple Imaging Detectors
Microscopy, dfaf022, https://doi.org/10.1093/jmicro/dfaf022
Published: 16 April 2025
Journal Article
ACCEPTED MANUSCRIPT
Mechanical characterization of nanomaterials revealed by Microscopic Nanomechanical Measurement method Get access
Microscopy, dfaf019, https://doi.org/10.1093/jmicro/dfaf019
Published: 07 April 2025
Image
Semi-automated image analysis using Stream Deck Plus. (a) Operational page ...
Published: 03 April 2025
Fig. 2.
Semi-automated image analysis using Stream Deck Plus. (a) Operational page for immunofluorescence image analysis. The bottom four circles indicate action wheels, which can be assigned multiple simple tasks or repetitive actions. (b) Part of the detailed settings for the key labeled with ‘file_mod DA
Journal Article
Semi-automated image acquisition and analyses for broad users utilizing macro keyboards
Microscopy, dfaf018, https://doi.org/10.1093/jmicro/dfaf018
Published: 03 April 2025
Image
Semi-automated immunofluorescence imaging using Stream Deck XL. (a) Operati...
Published: 03 April 2025
Fig. 1.
Semi-automated immunofluorescence imaging using Stream Deck XL. (a) Operational page for immunofluorescence imaging. (b–d) Detailed settings of multiple key actions. Custom macros were assigned to shortcuts as follows: selecting a 4× or 40× objective lens (4× or 40×) and DAPI or mCherry (Alt+Shift+M
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Semi-automated tissue imaging and analysis using Stream Decks. (a) Keys for...
Published: 03 April 2025
Fig. 3.
Semi-automated tissue imaging and analysis using Stream Decks. (a) Keys for tissue imaging. (b) Detailed setting of the multiple action key labeled with ‘JOBS Tissue 4×-10×’. JOBS is an automated acquisition pipeline tailored to user-specific projects. (c) Setting panel for designating the top, bott
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Physics related to interaction between electrons and sample in vEM. (a) Spe...
Published: 08 March 2025
Fig. 1.
Physics related to interaction between electrons and sample in vEM. (a) Spectrum of signal electrons. SE denotes secondary electron while BSE denotes backscattered electron. (b) The trajectory of PEs inside a specimen: from initial incidence to the emission of BSEs (magenta arrows), and the stopping
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Dependence of total electron emission yield (σ) on accelerating voltage. Ge...
Published: 08 March 2025
Fig. 2.
Dependence of total electron emission yield (σ) on accelerating voltage. Generally, to achieve weak charging and stable imaging for insulating specimens, it is appropriate to set the voltage slightly below V 2 , where the yield equals.
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Schematic illustrations of magnetic lenses: (a) Out-lens—the magnetic lens ...
Published: 08 March 2025
Fig. 4.
Schematic illustrations of magnetic lenses: (a) Out-lens—the magnetic lens effect is produced inside the lens due to the magnetic field formed between the inner (green) and outer yokes (magenta). (b) Semi-in-lens—the magnetic field is formed below the inner yoke (green), as the outer yoke (magenta)
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WD configurations for SEM imaging in SBEM, FIB/SEM, and array tomography: (...
Published: 08 March 2025
Fig. 6.
WD configurations for SEM imaging in SBEM, FIB/SEM, and array tomography: (a) WD in SBEM. (b) WD in FIB/SEM when alternating FIB cutting and SEM imaging at the co-incident point without stage movement. The process of secondary electron [SE (grey dotted arrow)] generation by backscattered electron [B
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Schematic illustration of various detection systems: (a) Traditional SE and...
Published: 08 March 2025
Fig. 7.
Schematic illustration of various detection systems: (a) Traditional SE and traditional BSE detectors placed inside the SEM chamber. (b) Through-the-lens (TTL) detector positioned laterally. (c) Doughnut-shaped TTL detector for a booster-type electrostatic lens. (d) Doughnut-shaped TTL detector for
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Angle change and linear flight length of electron in Monte Carlo calculatio...
Published: 08 March 2025
Fig. A1.
Angle change and linear flight length of electron in Monte Carlo calculation.
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The relationship between focal length (f) and the distance between...
Published: 08 March 2025
Fig. 3.
The relationship between focal length ( f ) and the distance between the center axis of the electron lens and the outer boundary of the primary electron beam. (a) For longer focal lengths, the distance between the center axis and the electron beam’s outer boundary is large. (b) For shorter focal len
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Schematic illustrations of electrostatic lenses: (a) a type of electrostati...
Published: 08 March 2025
Fig. 5.
Schematic illustrations of electrostatic lenses: (a) a type of electrostatic lens where an electrostatic potential is applied between the booster pipe and the exterior of the lens. The lens effect occurs in the lower portion of the electrostatic lens. (b) A type of electrostatic lens where an electr
Journal Article
Physical basics of scanning electron microscopy in volume electron microscopy
Microscopy, dfaf016, https://doi.org/10.1093/jmicro/dfaf016
Published: 08 March 2025
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Reconstructed 3D volume from the tilt series of a 300 nm lamella. The actua... Get access
Published: 04 March 2025
Fig. 3.
Reconstructed 3D volume from the tilt series of a 300 nm lamella. The actual thickness after reconstruction was about 360 nm. (a) An X - Y slice from the reconstructed volume. (b) The segmented volume, highlighting mitochondria and other membrane-bound organelles. The detailed morphology of the mi
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3D-reconstructed volume from thicker specimen which was ∼700 nm thickness. ... Get access
Published: 04 March 2025
Fig. 4.
3D-reconstructed volume from thicker specimen which was ∼700 nm thickness. The figure shows X - Y slices every 100 nm. After reconstruction, it was found that this position has a thickness of ∼700 nm. Nevertheless, membrane structure of mitochondria was still able to see, but the contrast become l
Journal Article
Cryo-STEM tomography for cell biology using thick lamella Get access
Microscopy, dfaf017, https://doi.org/10.1093/jmicro/dfaf017
Published: 04 March 2025
