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Siyuan Xia, Jun Wei, Jingya Wang, Huayan Sun, Wenting Zheng, Yangguang Li, Yanbo Sun, Huiyuan Zhao, Song Zhang, Ti Wen, Xinglong Zhou, Jian-Xin Gao, Puyue Wang, Zhenzhou Wu, Liqing Zhao, Zhinan Yin, A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells, Journal of Leukocyte Biology, Volume 95, Issue 5, May 2014, Pages 733–742, https://doi.org/10.1189/jlb.0713371
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ABSTRACT
Tregs (Foxp3+CD4+) are enriched in tumors to foster a tolerant microenvironment that inhibits antitumor immune response. IL-27 is reported to regulate the development and function of Tregs in vitro and in vivo; however, the effects of endogenous IL-27 on Tregs in the tumor microenvironment remain elusive. We demonstrated that in the absence of DC-derived IL-27, Tregs were decreased significantly in transplanted B16 melanoma, transplanted EL-4 lymphoma, and MCA-induced fibrosarcoma by using IL-27p28 conditional KO mice. Further studies revealed that IL-27 promoted the expression of CCL22, which is established to mediate the recruitment of peripheral Tregs into tumors. Tumor-associated DCs were identified as the major source of CCL22 in tumor sites, and IL-27 could induce CCL22 expression in an IL-27R-dependent manner. Intratumoral reconstitution of rmCCL22 or rmIL-27, but not rmIL-27p28, significantly restored the tumor infiltration of Tregs in IL-27p28 KO mice. Correlated with a decreased number of Tregs, tumor-infiltrating CD4 T cells were found to produce much more IFN-γ in IL-27p28 KO mice, which highlighted the physiological importance of Tregs in suppressing an antitumor immune response. Overall, our results identified a novel mechanism of action of IL-27 on Tregs in the context of cancers.
