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Lindsey R. Baden, William A. Blattner, Cecilia Morgan, Yunda Huang, Olivier D. Defawe, Magdalena E. Sobieszczyk, Nidhi Kochar, Georgia D. Tomaras, M. Juliana McElrath, Nina Russell, Kara Brandariz, Massimo Cardinali, Barney S. Graham, Dan H. Barouch, Raphael Dolin, the NIAID HIV Vaccine Trials Network 044 Study Team, Timing of Plasmid Cytokine (IL-2/Ig) Administration Affects HIV-1 Vaccine Immunogenicity in HIV-Seronegative Subjects, The Journal of Infectious Diseases, Volume 204, Issue 10, 15 November 2011, Pages 1541–1549, https://doi.org/10.1093/infdis/jir615
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Abstract
Background. To investigate the potential immunostimulatory effect of interleukin (IL) 2 as a human immunodeficiency virus type 1 (HIV-1) vaccine adjuvant, we conducted a study of a plasmid coding for a fusion protein of IL-2 and immunoglobulin (IL-2/Ig).
Methods. This phase I trial evaluated an HIV-1 DNA vaccine with the plasmid cytokine adjuvant (IL-2/Ig) in 70 HIV-negative adults. Subjects received placebo (group C), adjuvant alone (group A), vaccine alone (group D), increasing doses of adjuvant concurrent with vaccine (groups T1–T4), or adjuvant given 2 days after vaccine (group T5).
Results. No significant differences in adverse events were observed between treatment groups. Cellular immune responses to envelope protein EnvA peptides were detected by interferon (IFN) γ and IL-2 enzyme-linked immunospot (ELISPOT) assays in 50% and 40% of subjects, respectively, in T4, and in 100% and 80% in T5. The median responses for groups T4 and T5, respectively, were 90 and 193 spot-forming cells (SFCs)/106 peripheral blood mononuclear cells (P = .004; T4 vs T5) for the IL-2 ELISPOT assay and 103 and 380 SFCs/106 PBMCs (P = .003; T4 vs T5) for the IFN-γ ELISPOT assay. A trend to more durable cellular immune responses in T5 was observed at 1 year (T5 vs T4/D; P = .07). Higher anti-Env antibody responses were detected with T5 than with T4.
Conclusions. Plasmid IL-2/Ig significantly increased immune responses when administered 2 days after the DNA vaccine, compared with simultaneous administration. These observations have important implications for the development of cytokine augmentation strategies.
Clinical Trials Registration. NCT00069030.
- cytokine
- hiv
- plasmids
- aldesleukin
- immune response
- immunologic adjuvants
- antibody formation
- fusion proteins
- hiv-1
- interferons
- interleukins
- peptides
- vaccines
- dna vaccines
- immunoglobulins
- enzymes
- human leukocyte interferon
- enzyme linked immunospot assay
- peripheral blood mononuclear cell
- fifth thoracic vertebra
- immunogenicity, vaccine