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Anuja Mathew, Kim West, Siripen Kalayanarooj, Robert V. Gibbons, Anon Srikiatkhachorn, Sharone Green, Daniel Libraty, Smita Jaiswal, Alan L. Rothman, B-Cell Responses During Primary and Secondary Dengue Virus Infections in Humans, The Journal of Infectious Diseases, Volume 204, Issue 10, 15 November 2011, Pages 1514–1522, https://doi.org/10.1093/infdis/jir607
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Abstract
Low-avidity serotype–cross-reactive antibodies are hypothesized to play a key role in triggering severe disease in patients with secondary dengue virus (DENV) infection. However, there is little systematic information about the frequency, avidity, and cross-reactivity of DENV-specific B cells in individuals experiencing primary instead of secondary infection. We compared DENV-specific B-cell responses in a cohort of Thai children with primary or secondary DENV infection. B cells specific for DENV precursor membrane protein, envelope (E) protein, and nonstructural protein 1 were detectable in immune peripheral blood mononuclear cells with the highest frequencies of DENV E-specific B cells detected in patients experiencing primary DENV-1 infections. DENV E-specific B cells were highly serotype-specific after primary DENV infections, whereas most E-specific B cells in patients with secondary infection were serotype–cross-reactive and secreted antibodies with higher avidity to heterologous DENV serotypes. Our data suggest that the minor populations of serotype–cross-reactive B cells generated by primary DENV infection are preferentially expanded during secondary DENV infection.